EFFECT OF DIFFERENT HORMONAL CONCENTRATIONS ONIN-VITRO REGENERAT-ION OF RICE (Oryza sativaL.)

Abstract

The present study was aimed to evaluate the effect of different hormonal concentrations on the in vitro growth and multiplication of rice seeds. The experiment was performed in the tissue culture laboratory, department of biotechnology, UAJ&K Muzaffarabad, Azad Kashmir. Seeds were collected from the local market. Seeds were surface sterilized with different concentrations of Chlorox i. e. (30, 50 and 50 %) and germination percentage was calculated. The maximum (70 %) germination was recorded for seeds treated with 50% chlorox. Whereas minimum germination (37.5%) was recorded for seeds treated with 70% chlorox. The seeds were cultured on MS medium supplemented with varying concentrations of IAA (0.1, 0.2, 0.3, 0.75 and 2mg/l) and KIN at (0.5, 1, 1.5 mg/l). Maximum stem length (30.5 cm), root number (20), root length (6.6 cm), leaf number (70) and leaf length (7 cm) was recorded for MS medium supplemented with higher concentration of IAA (2mg/l) and KIN (1.5 mg/l). Minimum stem length (6.5 cm), root length (2.5 cm), root number (1) root length (2.5 cm), leaf number (1) and leaf length 1.7cm) was recorded on MS medium supplemented with IAA (0.3 mg/l) and KIN (1.5 mg/l). Results indicates that maximum germination percentage was observed in seeds treated with 50% chlorox and medium which was supplemented with high amount of IAA and KIN (2.0 mg/l, 1.5mg/l) significantly increase regeneration percentage.