ADVANCEMENT OF CROP PRODUCTIVITY VIA CRISPR-NANOPARTICLE INTERFACE

Amir Afzal; Sairah Syed; Mishal Khizar; Javed Iqbal; Sharmin Ashraf; Aneesa Altaf; Basharat Mehmood; Muhammad Rashid Khan

doi:10.34016/pjbt.2023.20.02.826

Authors

Amir Afzal Barani Agricultural Research Institute, Chakwal, Pakistan
Sairah Syed Barani Agricultural Research Institute, Chakwal, Pakistan
Mishal Khizar Barani Agricultural Research Institute, Chakwal, Pakistan
Javed Iqbal Barani Agricultural Research Institute, Chakwal, Pakistan
Sharmin Ashraf Barani Agricultural Research Institute, Chakwal, Pakistan
Aneesa Altaf Muhammad Nawaz Sharif University of Agriculture
Basharat Mehmood University of Poonch Rawalakot
Muhammad Rashid Khan Department of Physics, University of Poonch Rawalakot, AJK, Pakistan

DOI:

https://doi.org/10.34016/pjbt.2023.20.02.826

Keywords:

Precision, Plant Breeding, CRISPR, Gene Editing, Nanotechnology\

Abstract

Plant improvement strategies involve diverse techniques, ranging from traditional to marker-assisted methods, as well as chemical and radiation treatments. However, these methods can introduce imprecise changes in plant DNA. Accelerating plant enhancement is crucial to meet global food demand, but current methods are time-consuming. Scientists are revolutionizing plant breeding by employing various techniques to develop crops with specific attributes, such as increased yield and pest resistance, aligning with environmental and societal needs. While these methods offer substantial advantages, they often face challenges and can be less precise than desired. Innovative methods, such as gene editing using CRISPR, offer enhanced precision. CRISPR technology enables precise modifications to a plant's DNA, allowing for targeted improvements without unintended consequences. While CRISPR shows great potential, ensuring its safe and accurate implementation is a priority. Scientists are exploring diverse methods, both viral and non-viral, to effectively deliver CRISPR components into plant cells, with non-viral approaches gaining traction due to their safety and versatility. Nanoparticles play a pivotal role in these advancements by serving as delivery vehicles for CRISPR tools. These particles safeguard and transport the necessary components to specific locations within plants, bolstering growth, yield, and disease resistance. Despite challenges, the synergy of nanotechnology and CRISPR holds promise for revolutionizing plant improvement while safeguarding the environment. This integrated approach offers the potential to enhance crop growth and quality while upholding ecological balance.

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