EFFECT OF ANTIOXIDANTS TO CONTROL BROWNING IN GUAVA SOFT WOOD CUTTINGS

Mohsin Abbas; Komal  Aslam; Nida Mahreen; Maaz Aziz; Naseem Sharif; Qamar Shehzad Anjum; Muhammad Talha Bin Zahid

doi:10.34016/pjbt.2024.21.02.963

Authors

Mohsin Abbas Horticultural research institute, AARI, Faisalabad
Komal Aslam Scientific Officer Horticultural Research Institute, ayub agricultural Research institute, Faisalabad
Nida Mahreen Horticultural research institute, AARI, Faisalabad
Maaz Aziz Horticultural Research Institute, Ayub Agricultural Research Institute, Faisalabad, Punjab, Pakistan,
Naseem Sharif Horticultural research institute, AARI, Faisalabad
Qamar Shehzad Anjum Horticultural research institute, AARI, Faisalabad
Muhammad Talha Bin Zahid University of Agriculture, Faisalabad

DOI:

https://doi.org/10.34016/pjbt.2024.21.02.963

Keywords:

Citric acid, guava, browning, softwood cuttings, IBA

Abstract

Guava is the Apple of tropics and becoming the popular fruit of Pakistan. Guava is a nutraceutical fruit enriched with important minerals and vitamins like ascorbic acid. To overcome upon the issue of brown spots on fruit rind of guava soft wood cuttings, a research trial is executed. /the experiment was planned to overcome the problem of brown spots on fruit rind in soft wood cuttings of guava. For this purpose antioxidants i.e Vitamin C and L-ascorbate were used as blend mixture with IBA (rooting hormone) used for the initiation of roots in cuttings. A research trial was performed by employing randomized complete block design having three replications including frour treatments. Data subjected to result interpretation revealed that there is success (92.28%) and survival percentage (42.06%) was found maximum in T3 wherease, IBA 0.4% was used in combination with 1.5% citric acid and 1.5% ascorbic acid. In case of vegetative parameters maximum plant height (24.06 cm), number of leaves (25.50), leaf area (51.43mm), number of branches (3.68) and branch length (12.69 cm) were observed in T3 as compared to other treatments. The highest values for root parameters like primary roots (21.17), secondary roots (45.94), root volume (2.26 cm³) and root length (9.65 cm) were found in treatment 3 while lowest values for all treatments were observed in control.

Metrics

Metrics Loading ...

References

Abdelwahd, R., Hakam, N., Labhilili, M., & Udupa, S. M. (2008). Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of faba bean. African journal of biotechnology, 7(8).

Strosse, H., Van den Houwe, I., & Panis, B. (2004). Banana cell and tissue culture-review. Banana improvement: Cellular, molecular biology, and induced mutations, 1-12.

Rai, M. K., Asthana, P., Jaiswal, V. S., & Jaiswal, U. (2010). Biotechnological advances in guava (Psidium guajava L.): recent developments and prospects for further research. Trees, 24, 1-12.

Arafat, I., Hamed El-Sherif, A., & El-taweel, A. A. (2020). Effect of Pre-planting Treatments and Cutting Date on Performance of Guava (Psidium guajava L.) Semi-Hard Wood Cuttings. Scientific Journal of Agricultural Sciences, 2(2), 49-55.

Rahman, H. U., Khan, M. A., Khokhar, K. M., & Laghari, M. H. (1991). Effect of season on rooting ability of tip cuttings of guava (psidium-guajava) treated with paclobutrazol. Indian journal of agricultural sciences, 61(6), 404-406.

Gautam, N. N., Singh, K., Singh, B., Seal, S., Goel, A., & Goel, V. L. (2010). Studies on Clonal Multiplication of Guava ('Psidium guajava'L.) through Cutting under Controlled Conditions. Australian Journal of Crop Science, 4(9), 666-669.

N. Rahman, T. Ullah, G. Nabi and T. Jan, “Effect of different growth regulator and types of cuttings on rooting of guava (Psidium guajava L.)”, Quarterly Sci. Vision, vol. 9, pp. 1-2, 2002.

Rai, M. K., Jaiswal, V. S., & Jaiswal, U. (2009). Shoot multiplication and plant regeneration of guava (Psidium guajava L.) from nodal explants of in vitro raised plantlets. Journal of fruit and ornamental plant research, 17(1), 29-38.

Akram, M. T., Qadri, R. W. K., Imran Khan, I. K., Mohsin Bashir, M. B., Jahangir, M. M., Numra Nisar, N. N., & Khan, M. M. (2017). Clonal multiplication of guava (Psidium guajava) through soft wood cuttings using IBA under low-plastic tunnel. DOI: https://doi.org/10.17957/IJAB/15.0172

Shahzad, U., Kareem, A., Altaf, K., Zaman, S., Ditta, A., Yousafi, Q., & Calica, P. (2019). Effects of auxin and media additives on the clonal propagation of guava cuttings (Psidium guajava L.) var. Chinese Gola. J Agri Sci Food Res, 10(3), 265. DOI: https://doi.org/10.35248/2593-9173.19.10.265

Suttirak, W., & Manurakchinakorn, S. (2010). Potential application of ascorbic acid, citric acid and oxalic acid for browning inhibition in fresh-cut fruits and vegetables. Walailak Journal of Science and Technology (WJST), 7(1), 5-14.

El-Sharony, T. F., Rashedy, A. A., Abd Allatif, A. M., & Hassan, S. A. M. (2016, September). Stimulating rooting of guava cuttings by chemical and physical treatments. In VI International Symposium on Tropical and Subtropical Fruits 1216 (pp. 91-98). DOI: https://doi.org/10.17660/ActaHortic.2018.1216.12

Nitin, S., Pandey, S. K., Singh, S. S., Singh, S. R. K., Mishra, A., & Baghel, S. S. Kaurav (2015). Production of true to type guava plants through clonal propagation. International Journal of Applied and Pure Science and Agriculture (IJAPSA), 1(12), 131-136.

Wahab, F., Nabi, G., Ali, N., & Shah, M. (2001). Rooting response of semi-hardwood cuttings of guava (Psidium guajava L.) to various concentrations of different auxins. Online Journal of Biological Sciences, 1(4), 184-187. DOI: https://doi.org/10.3923/jbs.2001.184.187

Rahman, N., Nabi, T. G., & Taslim, J. (2004). Effect of different growth regulators and types of cuttings on rooting of guava (Psidium guajava L.). Quar. Sci. Vis, 91, 3-4.

Meahl, R. P. (1963). Influence of indigenous rooting factors and environmental on the seasonal function in root initiation of selected evergreen cuttings. In Proc. Amer. Soc. Hort. Sci (Vol. 83, pp. 811-813).

Vijaya Anand, A., Velayuthaprabhu, S., Rengarajan, R. L., Sampathkumar, P., & Radhakrishnan, R. (2020). Bioactive compounds of guava (Psidium guajava L.). Bioactive compounds in underutilized fruits and nuts, 503-527 DOI: https://doi.org/10.1007/978-3-030-30182-8_37

Samson, J. A. (1986). Tropical fruits 2nd ed. Trop. Agr. Ser., Longman Scientific and Technical, New York

Rathore, D. S. (1976). Effect of season on the growth and chemical composition of Guava (Psidium Guajava H.) fruits. Journal of Horticultural Science, 51(1), 41-47. DOI: https://doi.org/10.1080/00221589.1976.11514662

Feskanich, D., Ziegler, R. G., Michaud, D. S., Giovannucci, E. L., Speizer, F. E., Willett, W. C., & Colditz, G. A. (2000). Prospective study of fruit and vegetable consumption and risk of lung cancer among men and women. Journal of the National Cancer Institute, 92(22), 1812-1823. DOI: https://doi.org/10.1093/jnci/92.22.1812

Gordon, M. H. (1996). Dietary antioxidants in disease prevention. Natural product reports, 13(4), 265-273. DOI: https://doi.org/10.1039/np9961300265

Halliwell, B. (1996). Antioxidants in human health and disease. Annual review of nutrition, 16(1), 33-50. DOI: https://doi.org/10.1146/annurev.nutr.16.1.33

Singh, K. K. (2016). Rooting response of Guava (Psidium guajava L.) through cutting under Garhwal Himalayan region. Journal of Plant Development Sciences, 8(3), 167-169.

Jiménez-Escrig, A., Rincón, M., Pulido, R., & Saura-Calixto, F. (2001). Guava fruit (Psidium guajava L.) as a new source of antioxidant dietary fiber. Journal of Agricultural and food Chemistry, 49(11), 5489-5493. DOI: https://doi.org/10.1021/jf010147p

Khattak, M. S., Malik, M. N., & Khan, M. A. (2002). Guava propagation via in vitro technique.

Amin, M. N., & Jaiswal, V. S. (1987). Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell, Tissue and Organ Culture, 9, 235-243. DOI: https://doi.org/10.1007/BF00040809

Bisen, B. P. (2004). In vitro cloningof Brazilian Guava (Psidium guineense Sw.) (Doctoral dissertation, Ph. D. Thesis).

León de Sierralta, S., Arenas de Moreno, L., & Viloria, Z. (1997). Effect of exposure to sunlight of stock plants on the in vitro culture initiation of guava (Psidium guajava L.).

Meghwal, P. R., Sharma, H. C., Goswami, A. M., & Sritvastava, K. N. (2001). Effect of stock plant etiolation on in vitro phenol exudation during culture establishment of guava (Psidium guajava L.). Indian Journal of Horticulture, 58(4), 328-331.

Meghwal, P. R., Singh, S. K., & Sharma, H. C. (2003). Micropropagation of Aneuploid guava. Indian Journal of Horticulture, 60(1), 29-33.

Liu, X., & Yang, G. (2011). Clonal propagation of guava (Psidium guajava L.) on nodal explants of mature elite cultivar. International Journal of Plant Biology, 2(1), e2. DOI: https://doi.org/10.4081/pb.2011.e2

Zamir, R., Ali, N., Shah, S. T., Muhammad, T., & Shah, S. A. (2007). In vitro regeneration of guava (Psidium guajava L.) from shoot tips of mature trees. Pakistan Journal of Botany, 39(7), 2395-2398.

Zamir, R., Shah, S. T., Ali, N., Khattak, G. S. S., & Muhammad, T. (2004). Studies on in vitro surface sterilization and antioxidants on guava shoot tips and nodal explants. Pakistan Journal of Biotechnology, 1(2), 12-16.

Rahman, H. U., Khan, M. A., Khokhar, K. M., & Laghari, M. H. (1991). Effect of season on rooting ability of tip cuttings of guava (psidium-guajava) treated with paclobutrazol. Indian journal of agricultural sciences, 61(6), 404-406.

Gautam, N. N., Singh, K., Singh, B., Seal, S., Goel, A., & Goel, V. L. (2010). Studies on Clonal Multiplication of Guava ('Psidium guajava'L.) through Cutting under Controlled Conditions. Australian Journal of Crop Science, 4(9), 666-669.

Rai, M. K., Asthana, P., Jaiswal, V. S., & Jaiswal, U. (2010). Biotechnological advances in guava (Psidium guajava L.): recent developments and prospects for further research. Trees, 24, 1-12 DOI: https://doi.org/10.1007/s00468-009-0384-2