MOLECULAR DETECTION OF ENTAMOEBA GINGIVALIS USING POLYMERASE CHAIN REACTION

Abstract

Background and objective: Periodontitis is still until now not understood, it's being recorded as public health problems. This disease is caused by bacteria and parasite. Two parasites found in mouth Trichomonas tenax and Entamoeba gingivalis. Entamoeba gingivalis; opportunistic parasite finding in mouth, has only trophozoite form. This parasite possibly has a connection with Periodontitis. The objective of the study: compares between multi – methods for E. gingivalis detection, including microscopic examination, culturing the parasite in a modifying TrypticaseYeast Extract-Serum-Gastric Mucin-9 (TYSGM-9) medium and molecular detection of Polymerase Chain Reaction (PCR). Methods: 100 Cotton swab samples from patients with Periodontitis were collected using three or two sterile cotton swabs for everyone. The 50 samples were diagnosed by microscopic examination and 50 samples culturing on modifying TYSGM-9 media and then using PCR to insure found Entamoeba gingivalis grew on media and The 50 samples were diagnosed by PCR using specific primers of the SSU rDNA gene (Small Subunit of the ribosomal DNA gene). The No. of sample under testing 100 or 150 . Result: About 18 (36%) samples positive by microscope, and 20 (40%) samples positive by culture. Whereas the result of PCR positive for these 20samples of culture. So the PCR technique is the best method to detect E. gingivalis and modify TYSGM-9 media is perfect to grow this parasite. You mean all patients under study infections by parasite but no bacteria? Conclusions: The modifying TYSGM-9 medium and molecular detection by PCR using specific primers of the SSU rDNA gene are the best methods for E. gingivalis detection as ceasing to periodontal diseases and distinguished from other pathogens.