EVALUATION OF TRITICUM DURUM FOR SALT TOLERANT

Abstract

This work was conducted to evaluate salt tolerance in Triticum durum wheat varieties by selecting the high tolerant genotypes from the total genotypes under salt stress by using mature embryos. Mature embryos of four Triticum durum were grown on MS medium for germination under 4 concentrations of salt solutions (zero, 4000, 6000 and 8000ppm). The germination percent at the high salt concentration (8000ppm) was 77.7% S1, followed by77.1%D2, 66.2% ID12 and finally 64.5% D1 respectively. Plantlets on these media were transferred to pots with sand and irrigated with the same salt solutions. The produced grains were recultured to obtain some grains for propagation. Among the saline tolerance cultures proline accumulation was increased. SDS-page profile revealed an increasing in band intensity in 8000ppm than in the control and presented two bands with molecular weight 32and 28kda related to proline accumulation in the cells. The four Triticum durum (wheat) varieties had the ability to resist against salt stress.