OPTIMIZATION OF CULTURE CONDITION FOR PROTEASE PRODUCTION BY ASPERGILLUS NIGER

Abstract

Proteases are used in various biotechnological industries such as detergent, food,
pharmaceutical and cosmetics. Due to high demand, the production of protease by Aspergillus
niger was studied on the basis of various fermentation parameters such as incubation time, carbon
sources, nitrogen sources, pH and temperature. On the basis of present study, it was noted that
Aspergillus niger secreted higher yield of protease (0.131mg/ml) when grown on mineral medium
containing 0.3% glucose and 0.02% yeast extract as a carbon and nitrogen sources in comparison to
other carbon and nitrogen sources at 30±2°C for 96 hour. Whereas pH 7.0 and 35°C was found
favorable and the yield of protease increased up to 0.199 mg/ml by Aspergillus niger.