CELLULASES PRODUCTION BY ASPERGILLUS NIGER GROWN ON MEDIUM CONTAINING ACID PRETREATED AGRO WASTES AS CARBON SOURCE.

Abstract

In this study agricultural wastes such as sugarcane peeler bagasse, sugarcane bagasse of industry, banana
fruit stalk, sorghum husk and rice husk were hydrolyzed with 0.6N HNO3 and the fermentable sugars were
incorporated in mineral medium as a carbon source for the growth of Aspergillus niger and production of
cellulases using submerged fermentation method. The maximum production (10.89 units/ml) of CM-cellulase was
obtained at 240 hours of incubation when banana fruit stalk hydrolysate was used as a carbon source. The
cellobiose and salicinase maximum production 4.855 and 4.198 units/ml were obtained at 24 and 240 hours
respectively when sugar cane peeler and rice husk hydrolysate used as a carbon source. Maximum cell growth
0.402g/100ml of Aspergillus niger was observed at pH 7.62 in case of hydrolyzed rice husk used as a carbon
source. The total sugar and reducing sugars were decrease with the growth of Aspergillus niger in all carbon
sources..