TOXICOLOGICAL EFFECTS OF TERTIARY MIXTURE OF METALS ON THE OXIDATIVE STRESS IN CIRRHINA MRIGALA AND LABEO ROHITA

Javiria Aslam; Muhammad  Javed; Fariha Latif; Safina Kousar; Rehana Iqbal

doi:10.34016/pjbt.2023.20.01.280

Authors

Javiria Aslam Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad.
Muhammad Javed Institute of Zoology, Bahauddin Zakariya University, Multan.
Fariha Latif Institute of Zoology, Bahauddin Zakariya University, Multan.
Safina Kousar Institute of Zoology, Bahauddin Zakariya University, Multan.
Rehana Iqbal Institute of Zoology, Bahauddin Zakariya University, Multan.

DOI:

https://doi.org/10.34016/pjbt.2023.20.01.280%20

Keywords:

antioxidant enzymes, superoxide dismutase, Major carps

Abstract

Present research work was conducted to measure the effects of tertiary metals mixture (Fe+Zn+Mn) on superoxide dismutase (SOD) activity in various tissues of two major carps, Cirrhina mrigala and Labeo rohita at controlled laboratory conditions. 90-day-old fingerlings of both fish species were exposed to 1/4^th and 1/5^th of their respective 96-hr LC₅₀value of Fe+Zn+Mn mixture, for 24 days. After 6, 12, 18, and 24-day exposure, fish from each treatment will be sampled, dissected and their tissues viz. brain, gills, kidney, and heart isolated for the SOD enzyme assay. The physical and chemical parameters of test media viz. pH, temperature, dissolved oxygen, total hardness, carbon dioxide, total ammonia, magnesium, and calcium were determined on a 12-hourly basis. It was observed that with an increase in metal concentration, the activity of enzymes increased significantly in both fish species which was maximum at 1/4^th of LC₅₀with the mean value of 49.35±10.04 UmL^-1in C. mrigala. In Labeo rohita, SOD activity decreased with an increase in exposure duration. SOD activity was maximum on day 6 at 52.22±12.91 UmL^-1, and on day 24, it was minimum at 35.01±6.91 UmL^-1. Among the organs, the SOD activity followed the trend: gills > heart > kidney > brain. The various tissues of metals mixture treated fish Cirrhina mrigala showed significantly increased activity of SOD in comparison to Labeo rohita. All the physicochemical parameters varied significantly at p<0.05 during this study period.

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References

A.P.H.A. (American Public Health Association). Standard methods for the examination of water and wastewater. (22nd Ed.) American Public Health Association, New York, p. 1360. (2012)

Abdullah, S., M. Javed and A. Javid. Studies on acute toxicity of metals to the Fish (Labeo rohita). Int. J. Agric. Bio., 2: 333-337. (2007)

Avci, A., M. M. Kacmaz and I. Durak. Peroxidation in muscles and liver tissues from fish in a contaminated river due to a petrolium refinery industry. Ecotoxicol. Environ. Saf., 6:101-105. (2005)

Azmat, H., M. Javed and G. Jabeen. Acute toxicity of aluminium to the fish (Catla catla, Labeo rohita and Cirrhina mrigala). Pak. Vet. J., 32: 85-87. 2012 Barata, C., D. J. Baird, A. J. A. Nogueira, A. M. V. M. Soares and M. C. Riva. Toxicity of binary mixtures of metals and pyrethroid insecticides to Daphnia magna Straus. Implications for multi-substance risks assessment. Aquat. Toxicol., 78: 1-14. (2006)

Batool, M., S. Abdullah and K. Abbas. Antioxidant enzymes activity during acute toxicity of chromium and cadmium to Channa marulius and Wallago attu. Pak. J. Agri. Sci., 51: 1017-1023. (2014)

Basha, P. S. and A. U. Rani. Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus. Ecotoxicol. Enviorn. Saf., 56: 218-221. (2003)

Bhattacharya, A. and S. Bhattacharya. Induction of oxidative stress by arsenic in Clarias batrachus: Involvement of peroxisomes. Ecotoxicol. Environ. Saf., 66: 178-187. (2007)

Chen, M., G. Cheng, C. Lin, B. Chen and Y. Huang. Trace minerals in fish nutrition. Biol. Trace Elem. Res., 110: 163-177. (2006)

Emerit. J., M. Edeas, F. Bricaire. Neurodegenerative diseases and oxidative stress. Biomed Pharmacother, 58: 39-46 (2004)

Fraga, C. G. and P. I. Oteiza. 2002. Iron toxicity and antioxidant nutrients. Toxicology, 180: 23-32.

Frazzini V., E. Rockabrand, E. Mocchegiani and S. L. Sensi. Oxidative stress and brain aging: is zinc the link?

Biogerontology, 7: 307-314. (2006)

Gagnaire, B., H. T. Guyon and T. Renault. In vitro effects of cadmium and mercury on Pacific oyster, Crassostrea gigas (Thunberg), haemocytes. Fish & Shellfish Immunology. 16: 501-512. (2004)

Giannopolitis, C. N. and S. K. Ries. Superoxide dismutase occurrence in higher plants. Plant Physiol., 59: 309-

(1977)

Hansen, B. H., S. Romma, O. A. Garmo, S. A. Pedersen, P. A. Olsvik and R. A. Andersen. Induction and activity of oxidative stress-related proteins during waterborne Cd/Zn-exposure in brown trout (Salmo trutta). Chemosphere, 67: 2241-2249. (2007) Hassan, W., S. Abdullah, K. Abbas, M. Batool and S. Yaqub. The effect of sub-lethal level of dietary copper and cadmium on the growth performance and oxidative stress in Cirrhina mrigala. Pak. J. Agri. Sci., 52: 789-794. (2015)

Jezierska, B. and M. Witeska.. Summary of metal-induced disturbance in fish organism. In: metal toxicity to fish. Fish Physiol Biochem., 2: 214-243. (2001) Khalid, M. U., N. A. Qureshi, M. S. Mubarik and S. A. Bukhari. Heavy metals (chromium, copper and cadmium) induced oxidative stress in Labeo rohita (Hamilton 1822) during acute and chronic toxicity experiment. Int. J. Biosci., 6: 64-72. (2015)

Khayatzadeh, J. and Abbasi, E. The effects of heavy metals on aquatic animals. The 1st international applied geological congress, department of geology, Islamic Azad University, Mashad Branch, Iran. 688-694. (2010)

Lange, A., O. Ausseil and H. Senger. Alteration of tissue glutathione level and metallothione mRNA in rainbow trout during single and combined exposure to cadmium and zinc. Comp. Biochem. Physiol., 131: 231-243. (2002)

Li, H., Q. Zhou, Y. Wu, J. Fu, T. Wang and G. Jiang. Effects of waterborne nano-iron on medaka (Oryzias latipes): Antioxidant enzymatic activity, lipid peroxidation and histopathology. Ecotoxicol. Environ. Saf., 72: 3684-3692. (2009)

Liu, H., X. R. Wang, W. M. Wang and H. Shan. Effects of long-term exposure of low level zinc and Zn-EDTA complex on zinc accumulation and antioxidant defense system in liver of Carassius auratus. J. Environ. Sci., 26: 173-176. (2005)

Loro, V. L., M. B. Jorge, K. R. D. Silva and C. M. Wood. Oxidative stress parameters and antioxidant response to sub-lethal waterborne zinc in a euryhaline teleost Fundulus heteroclitus: Protective effects of salinity. Aquat. Toxicol., 110: 187-193. (2012)

Madhavan, P. and K. Elumalai. 2016. Effects of chromium (VI) on the lipid peroxidation and antioxidant parameters in the gill and kidney tissues of catfish, Clarias batrachus . (Actinopterygii: Siluriformes). Int.

J. Adv. Res. Biol. Sci., 3: 249-255. (Linnaeus, 1758)

Mohanty, B. P., M. R. Mahananda and S. Pradhan.

Cadmium Induced Toxicity and Antioxidant

Activities in Labeo rohita (Hamilton). Environ. Ecol., 1: 41-47. (2013)

Muyssen, B. T. A., K. A. C. D. Schamphelaere and C. R. Janssen. Mechanisms of chronic waterborne Zn toxicity in Daphnia magna. Aquat. Toxicol., 77: 393-

(2006)

Naz, S. Growth responses and metal accumulation patterns in fish exposed to chronic metal mixture concentrations. Ph.D. thesis, Department of Zoology and Fisheries, University of Agriculture, Faisalabad,

Pakistan. (2013)

Owa, F. D. Water pollution: Sources, effects, control and management. Medit. J. Social Sci., 4: 65-68. (2013)

Oze, G., R. Oze, C. Anunuso, C. Ogukwe, H. Nwanjo and K. Okorie. Heavy metal pollution of fish of Qua-Iboe river estuary: Possible implications for neurotoxicity. Int. J. Toxicol., 3: 1-6. (2005)

Pandey, S., S. Parvez, R. A. Ansari, M. Ali, M. Kaur, F. Hayat, F. Ahmad, S. Raisuddin. Effects of exposure to multiple trace metals on biochemical, histological and ultrastructural features of gills of a freshwater fish, Channa punctata Bloch. Chem. Biol. Interact., 174: 183-192. (2010)

Parthiban, P. and M. Muniyan. Effects of heavy metal the lipid peroxidation and antioxidant parameters in the liver tissue of Cirrhinus mrigala (HAM). Int. J. Development Res., 1: 1-4. (2011)

Rajkowska, M. and M. Protasowicki. Distribution of metals (Fe, Mn, Zn, Cu) in fish tissues in two lakes of different trophy in Northwestern Poland. Environ. Monit. Assess., 185: 3493-3502. (2013).

Rashed, M. N. Cadmium and lead levels in fish (Tilapia nilotica) tissues as biological indicator for lakewater pollution. Environmental Monit. Assess., 68: 75–89. (2001)

Rauf, A., M. Javed, M. Ubaidullah and S. Abdullah. Assessment of heavy metals in sediments of River Ravi, Pakistan. Int. J. Agric. Biol., 11: 197-200. (2009)

Soundararajan, M., G. Veeraiyan and S. S. Samipillai. Arsenic-induced oxidative stress in fresh water catfish Tilapia mossambica. J. Phytol. 1: 267-276. (2009)

Valko, M., H. Morris and M. T. D. Cronin. Metals, toxicity and oxidative stress. Curr. Med. Chem., 12: 1161-

(2005)

Vinodhini, R. and M. Narayanan. Biochemical changes of antioxidant enzymes in common carp (Cyprinus carpio L.) after heavy metal exposure. Turk. J. Vet. Anim. Sci., 33: 273-278. (2009)

Zikic, R. V., A. S. Stajn, S. Z. Pavlovic, B. I. Ognjanovic and Z. S. Saicic. Activities of superoxide dismutase and catalase in erythrocytes and plasma transaminases of goldfish (Carassius auratus) exposed to cadmium. Physiol. Res, 50: 105-111. (2001)