GENETIC TRANSFORMATION OF POTATO (Solanum tuberosum L. ) Cv. NOOKSACK WITH FBPase/ClRan1 GENES MEDIATED BY Agrobacterium tumefaciens.

Abstract

Nooksack is one of potential cultivar to be used as french fries material. Fructosa 1.6-bisphosphatase (FBPase) and
Citrullus lanatus Ran1(ClRan1)genes play a very important role by increasing potato productivity. Overexpression of FBPase
gene is able to accelerate photosynthesis whereas overexpression of ClRan1 genesis to develop the stolon. This study aimed to
introduce FBPase/ClRan1 genes into the genome of potato cv. Nooksack used Agrobacterium tumefaciens. Leaf disc and
internode of potato was inoculated with A. tumefaciens EHA105 bringing FBPase/ClRan1 genes under control of rbsS3C
promoter for FBPase gene and 35SCaMV promoter for ClRan1 gene. Leaf discs have a transformation efficiency (83.33%) and
regeneration efficiency (16%) better than internode with transformation efficiency (50%) and regeneration efficiency (6.66%).
PCR analysis indicated that FBPase/ClRan1 genes has been integrated into potato with size of the FBPase/CLRan1 genes was
1047 bp. Data analysis used SPSS 16 with one way ANOVA method on the level of 95%. FBPase/ClRan1 gene was
significantly different from the photosynthesis rate, leaf number, plant height, stem diameter, the number of bulbs and tuber
weight. Whereas, the wet weight and dry weight of shoots were not significantly different.