Introduction of Hd3a gene in IPB CP1 potato cultivar through Agrobacterium tumefaciens-mediated transformation under the control of use 35S CaMV promoter

Abstract

IPB CP1 (Chip potato 1) potato cultivar is a new potato varieties obtained by irradiating the Atlantic potatoes at a dose of
fifteen gray by the Indonesian National Atomic Energy Agency (BATAN) and researchers at the Bogor Agricultural University.
In Indonesia, these potatoes are used as chips. Tuber is a plant organ that serve as storage organ for nutrient and it is used for
survival during dry season. One way to increase production of potato is through induction of its tuber. Heading date gene 3a
(Hd3a) is one of the genes that can regulate flowering time in rice. In addition to inducing flowering, this gene have proven to
induce the formation of tuber in Andigena potato cultivar. In this study we introduced Hd3a gene under the control of 35S CaMV
promoter into the IPB CP 1 potato cultivar to induce formation of its tubers. Genetic transformation was done by co-cultivation
method using Agrobacterium tumefaciens strain LBA4404 carrying Hd3a gene. From the transformation of 157 explants from
internodes, we obtained 16.4% and 23.1% as value of transformation efficiency and regeneration efficiency respectively. Six
putative transgenic shoots survive on callus induction medium containing 15mg/L of hygromycin antibiotic. Molecular analysis
was carried out by using 35S-F primers and Hd-R primers to prove if Hd3a gene was integrated into IPB CP 1 potato. PCR
analysis showed that all of the six putative transgenic IPB CP 1 potato contained the Hd3a gene under the control of 35S CaMV
promoter.