PHYTOGENOTOXICITY ASSESMENT OF MOSQUITO REPELLENT COILS USED IN PAKISTAN VIA PLANT CHROMOSOMAL ABERRATION ASSAY

Sadaf Tabasum Qureshi; Asma Parveen Channar; Anila Naz  Soomro

doi:10.34016/pjbt.2024.21.02.928

Authors

Sadaf Tabasum Qureshi Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan https://orcid.org/0000-0003-0132-9951
Asma Parveen Channar Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan
Anila Naz Soomro Department of Fisheries and Aquatic Sciences University of Sindh, Jamshoro

DOI:

https://doi.org/10.34016/pjbt.2024.21.02.928

Keywords:

mosquito repellent coils, Genotoxicity, Cytotoxicity, chromosomal aberrations

Abstract

The rising prevalence of mosquitos borne deadly illnesses has made mosquitoes repellent usage common in Pakistan, with coils the popular choice. In order to estimate safe limits for humans three most commonly used type of mosquito repellent coils in Pakistan were compared for phyto-genotoxicity (DNA damage) using chromosomal aberration assay in Chickpea (Cicer arietinum L.) root tip cells. Chickpea seeds per treatment were incubated in coil smoke of five brands (PPJ, KRD, KCEP, GNA, TL) for 20, 40 and 60 minutes in approximately 24 by 24 inch Paper carton. Germination percentage was recorded by sowing seeds in sand pots. Genotoxic effects were assessed using a plant chromosomal aberration assay, measuring the Mitotic index, abnormality index and types of chromosomal aberrations were recorded to categorize different repellents. All the incubations of coils inhibit germination of chickpea seed as compared to positive control. The inhibitory effects on germination were more pronounced as the incubation increases except PPJ. For Mitotic index highly significant variation (p≥0.01) were found among all coils and their doses (20, 40, 60 minutes). KRD exhibited non-significant variations for only 60 minutes incubation. Decreasing trends for mitotic index with increasing doses was observed for most of treatments. For abnormality index highly significant variation (p≥0.01) were found among all the doses for KRD, where as PPJ and TL exhibited non-significant variations between 40 and 60 minutes incubation. Based on the Mitotic Index and abnormality index results, KCEP and KRD coils demonstrated highest genotoxicity. Therefore, these brands of coils should not be used and other brands may also be avoided

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References

Al-Ahmadi, M.S., (2013). Effects of organic insecticides, Kingbo and Azdar 10 EC, on mitotic chromosomes in root tip cells of Allium cepa. L. International Journal of Genetics and Molecular Biology. 5(5):64-70.

Abdul-Jabbar, (1992). In: Pesticide Poisoning In Humans . Published by. National Agricultural Research Centre, Islamabad. p. 251-255.

Al-Saleh, I.A., (1994). Pesticides: a review article. Journal of Environmental, Pathology, Toxicology and Oncology. 13(3):151-61.

Amer, S.M., Ibrahim, A.A.E., Aly, & F.A.E., (1998). Induction of Chromosome Aberration in Mouse Germ Cells by the Organophosphorus insecticides Dursban and Dichlorvos and sperm abnormalities in the treated Mice. Scientific Medical Journal of Egyptian Society for Continued Medical Education. 10(1), 97-107.

Ananthakrishnan, M., Kumarasamy, K., &Antony, A.S. (2013). Genotoxic effects of Furadan and Endosulphan on (Allium cepa) root tips. Asian Journal of Pharmaceutical and Clinical Research. 6(1):126-131.

Anonymous, (1991). Bioallethrin. International Program of Chemical Safety (IPCS), Commission of the European Communities, WHO, 2920 Luxenburg, Grand Duchy of Luxenburg, Geneva 27, Switzerland, 1211 pp.

Ayla, C., Oya, O. & Ulku, C., (2005). The evaluation of micronucleus frequency by Acridine Orange fluorescent staining in peripheral blood of rats treated with lead acetate. Mutagenesis. 20: 411-415.

Azizi, B.H.O. & Henry, R.L. (1991). The effects of indoor environmental factors on respiratory illness in primary school children in Kuala Lumpur. International Journal of Epidemiology. 20(1):144–149.

Chang, J. & Lin, J. (1998). Aliphatic aldehydes and allethrinin mosquito coil smoke. Chemosphere. 36(3):617–624.

Doll, R. & Peto, R., (1981). The causes of cancer: quantitative estimates of avoidable risk of cancer in the United States today. New York: Oxford University Press.

Dubey, K.K. & Fulekar M.H. (2011). Effect of pesticides on the Seed Germination of Cenchrus setigerus and Pennisetum pedicellatum as Monocropping and Co-cropping System:Implications for Rhizospheric Bioremediation. Romanian Biotechnological Letters. 16(1): 5909-5918.

Fagbule, D. & Ekanem, E.E. (1994). Some environmental risk factors for childhood asthma: a case-control study. Annals of Tropical Pediatrics. 14(1):15–19.

Farrell, C. L. and Risau, W. (1994). Normal and abnormal development of the blood–brain barrier. Microscopy Research and Technique. 27:495–506.

Galloway, S.M. (1994). Genotoxicity testing. Mutation Research. 312:195-322.

Garg, P. & Garg, P, (2004). Mosquito Coil (Allethrin) Poisoning on two brothers. Indian Pediatr. 41(11):1177-8.

Grant, W.F. (1978). Chromosome aberration in plants as monitoring system. Environmental Health Perspective. 27, 4-7.

Gul, S., Ibrahim, S., Wasif, N., Zafar, A., & Syed, R., (2013). Mosquito repellents: Killing mosquitoes or yourselves. Journal of Scientific and Innovative Research. 2(6): 1052-1057.

Kamble, V.S. (2012). Study of chronic treatment of mosquito repellent liquid inhalation on biochemical on constituents of rat. International Journal of Applied Biology and Pharmaceutical Technology. 3(4): 189-192.

Karim, M.R., Dipayon, G., Krisna, F., Rahman, T., Hossain, A. Rahman, R. & Islam (2020). Evidence of health complications caused by mosquito coil smoke inhalation in mouse model. Journal of Advanced Biotechnology and Experimental Therapeutics. 2:122-127.

Kengar, Y.D., Kamble, A.B. & Sabale, A.B. (2014). Effect of hexaconazole and triazophos on seed germination and growth parameters of spinach and gaur. Annals of Biological Research. 5(5):89-92.

Koo, L.C.L. & Ho J.H.C., (1994). Mosquito coil smoke and respiratory health among Hong Kong Chinese epidemiological studies. Indoor Environmental. 3:304–310.

Leng, G., Kuhn, K.H., Wieseler, B. & Idel, H., (1999). Metabolism(s) of bioallethrin and related compounds in human. Toxicological Letters. 107:109– 121.

Liu, W., Zhang, J., Hashim, J.H., Jalaludin, J., Hashim, Z. & Goldstein, B.D. (2003). Mosquito Coil Emissions and Health Implications. Environmental Health Perspectives. 111: (12), 1454-1460.

Lukwa, N. & Chandiwana, S.K., (1998). Efﬁcacy of mosquito coils containing 0.3% and 0.4% pyrethrins against An. Gambiae sensulato mosquitoes. Central African Journal of Medicine. 44(4):104–107.

Mark, S., Fradin, M.D. & Day, J.F. (2002). Comparative Efficacy of Insect Repellents against Mosquito Bites, New England Journal of Medicine, 347:13-18.

M-boh, (2003). Genotoxicity: There should or not, introduction of cases of industries chemicals. Toxicology Letters. 140-141.

Mishra, K. P. (2004). Cell membrane oxidative damage induced by gamma-radiation and apoptotic sensitivity. Journal of Environmental Pathology and Toxicology. 23:61-66.

Narendra, M., Kavitha, G., Kiranmai, A.H., Rao, N.R., Varadacharyulu, N.C. (2008). Allethrin-induced biochemical changes and properties of human erythrocyte membrane. African Journal of Biochemistry Research. 2(1): 024-029.

Narendra, M., Kavitha, G., Kiranmai, A.H., Rao, N.R. & Varadacharyulu, N.C. (2008). Chronic exposure to Pyrethroid-based Allethrin and prallethrin mosquito repellents alters plasma biochemical profile. Chemosphere.73:360–364.

Nerio, L.S., Olivero-Verbel, J. & Stashenko, E. (2010) Repellent activity of essential oils: A review. Bioresource Technolgy.101: 372-378.

Noman, S.A., Qureshi, S.T., Hassaney, S.S. and Soomro, A.N. (2022). Infertility curative plant growth inhibitory agents. Pakistan Journal of Botany. 54(6):2179-2186.Noreena, (2024). Genetic Toxicology of different weight loss medicinal plants in onion (Allium cepa L.). (M. Phil dissertation, University of Sindh Jamshoro).

Panwar, M., Usha, G. & Kumath, M. (2013). Status epilepticus: An association with pyrethroid poisoning. Indian Journal of Critical Care Medicine. 17(2):119-20.

Patel, E.K., Gupta, A. & Oswal, R.J., (2012). A Review On: Mosquito Repellent Methods. International Journal of Pharmaceutical, Chemical and Biological Sciences. 2(3): 310-317.

Proudfoot, A.T., (2005). Poisoning due to pyrethrins. Toxicological Reviews; 24:107-13.

Racuciu, M., 2009. Effect of radiofrequency radiation on root tip cells of Zea mays. Romanian Biotechnologycal Letters. 14(3), 4365–4369.

Renjana, P.K., John, E. & Thoppil, (2013). Toxicological Evaluation of Root Methanolic Extract of Strobilanthes heyneanus Nees Using Allium Test. International Journal of Pharmaceutical Sciences and Drug Research. 5(3): 125-128.

Riss, T.L. & Moravec, R.A. (February 2004). Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay Drug Development Technologies. 2(1): 51–62.

Sinha, C., Salh-K, Islam, F., Chatnrvedi, R.K., Shukla, S., Mathur, N., Srivastava, N. & Agerwal, A.K. (2006). Behavioral and Naurochemical effects induced by pyrethroid based mosquito repellent exposure in rat offsprings during parental and early postnatal period. Neurotoxicology and Terotology, 28:472-481.

Stahl, A. (2002). The Health Effects of Pesticides Used for Mosquito Control. A report is a product of Citizens Campaign for the Environment (CCE) and Citizens Environmental Research Institute (CERI). Edt. Meyl and SJ.p. 1-15.

Tawatsin, A., Wratten, S.D. & Scott. R.R. (2001). Repellency of volatile oils from plants against three mosquito vectors. Journal of Vector Ecology. 26: 76-82.

Verma, R., Awasthi, K.K., Soni, I. & John, P.J. (2013). Evaluation of Cytogenetic Effects of β-Cyfluthrin in Swiss Albino Mice. International Journal of Current Microbiology and Applied Sciences. 2(6):30-40.

Victoria, M., Debra, E.L. & Kellie, J. (2013). Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia. Nursing Research and Practice. Pp. 1- 12.

Yap, H.H., Lee, C.Y. & Chong, N.L. (1996). Performance of mosquito coils containing transfluthrine against Culexquinque fasciatus an urban squatter environment. Tropical Biomedicine. 13:101-103.

Yap, H.H., Tan, H.T. & Yahaya, A.M. (1990). Field efficacy of mosquito coil formulations containing d-allethrin and d-transallethrin against indoor mosquitoes especially Culexquinquefasciatus. Southeast Asian Journal of Tropical Medicine and Public Health. 21: 558-563.

Yuzbaşioğlu, D., Unal, F. & Sancak, C. (2009). Genotoxic effects of herbicide Illoxan (Diclofop-Methyl) on Allium cepa L. Turkish Journal of Biology. 33: 283-290

Zeljezic, D. & Vrhovac, V.G. (2001). Chromosomal Aberration and Single cell gel electrophoresis (comet) assay in the longitudinal risk assessment of occupational exposure to pesticides. Mutagenesis. 16(4):359-363.

Zhang, W., Sun, J., Chen, S., Wu, Y. & He. F. (1991). Levels of exposure and biological monitoring of pyrethroids in spray men. British Journal of Industrial Medicine. 48:82–86