EFFECTS OF GROWTH REGULATOR ON INVITRO PROPAGATION OF LILIUM USING BULB SCALE
DOI:
https://doi.org/10.34016/pjbt.2024.21.02.927Keywords:
Lily, Horticultural crop, Micropropagation, Phytohormones, Tissue cultureAbstract
Lilium spp. is the most significant decorative plant and has high demand in the floriculture market. We studied the impact of different growth regulators on culture initiation, shoot proliferation, and root formation of Lilium for disease-free plants from in vitro propagation. The explant basal scale was used for culture initiation, and varying concentrations of BAP (6-Benzylaminopurine) alone and in combination with IAA (Indole-3-acetic acid) showed different responses. It was observed that MS media supplemented with BAP at 1.5 mg/l and IAA at 1.0 mg/l exhibited the maximum number of shoots, i.e., 4.3 shoots and 7 cm length of shoots, which were attained in four weeks. Several concentrations of BAP and Kinetin (KIN) supplemented with MS medium alone or in combination with BAP with IAA, IBA, and NAA were used for shoot proliferation. The results revealed that all treatments resulted in 100% shoot development, with significant variability in the number of shoots and shoot length. MS supplemented with 1.5 mg BAP and 1.0 mg KIN showed the maximum 7.4 number of shoots, with an average shoot length of 7.7 cm. The maximum 11 roots observed in MS supplemented with 1.5mg IBA and the longest roots 4.4cm found in MS with 2mg IAA, and the rooted plantlets were hardened in peat moss media, had the highest ex-vitro survival rate. These results highlight the significance of optimized combinations of growth regulators for improving micropropagation efficiency in the horticulture industry.
Metrics
References
Arbaoui, S., Soufi, S., & Bettaieb, T. (2017). Effects of β-cyclodextrin on in vitro rooting and bulbing of lilium (Lilium longiflorum L.). Int. J. Horticultural Sci. Ornamental Plants, 32(2), 59-63.
Askari, N., Visser, R. G., & De Klerk, G.-J. (2018). Growth of lily bulblets in vitro, a review. International Journal of Horticultural Science and Technology, 5(2), 133-143.
Bhandari, N. S., Aswath, C., Bakshi, M., & Goshwami, V. (2019). Shoot regeneration and bulblets development from in vitro leaf petioles of La hybrid lily “Pavia”. Int J Agr Stat Sci, 15, 801-806.
Cui Qi, C. Q., & Jia GuiXia, J. G. (2014). Optimization of tissue culture and rapid propagation system for Lilium.
Gochhayat, A. A., Beura, S., & Rout, S. STANDARDIZATION OF IN VITRO REGENERATION OF HYBRID LILIUM Cv. TRESOR.
Gochhayat, A. A., Beura, S., & Subudhi, E. (2017). Effect of surface sterilization time and plant bioregulators for callus formation in hybrid Lilium Cv. Tresor. Biosciences Biotechnology research Asia, 14(2), 709-713. DOI: https://doi.org/10.13005/bbra/2498
Huong, B. T. T., Gioi, D. H., & Van Thang, B. (2017). Optimisation of an in vitro propagation protocol for a valuable lily (Lilium spp.). Journal of forestry science and technology (5), 018-025.
Kakani, A., Li, G., & Peng, Z. (2009). Role of AUX1 in the control of organ identity during in vitro organogenesis and in mediating tissue specific auxin and cytokinin interaction in Arabidopsis. Planta, 229, 645-657. DOI: https://doi.org/10.1007/s00425-008-0846-6
Kaur, R., Thakur, N., & Sharma, D. (2006). Low cost strategy for micropropagation of Lilium Asiatic hybrid cv. Toscana. Journal of Horticultural Sciences, 1(1), 24-27. DOI: https://doi.org/10.24154/jhs.v1i1.668
Khan, S., Jaskani, M. J., Iqbal, M. Z., & Rafiq, A. (2015). Rapid multiplication of ornamental bulbous plants of Lilium orientalis and Lilium longiflorum. International Journal of Modern Agriculture, 4(4), 57-61.
Kumar, A., & Dogra, I. (2020). In vitro micropropagation of calla lily: an Overview. Indian Journal of Pure and Applied Biosciences, 8(2), 144-153. DOI: https://doi.org/10.18782/2582-2845.7974
Lapiz-Culqui, Y. K., Meléndez-Mori, J. B., Mállap-Detquizán, G., Tejada-Alvarado, J. J., Vilca-Valqui, N. C., Huaman-Human, E., . . . Goñas, M. (2022). In vitro bulbification of five lily varieties: an effective method to produce quality seeds and flowers. International Journal of Agronomy, 2022. DOI: https://doi.org/10.1155/2022/8775989
Li, Y., Xia, X., Liang, J., & Yang, Z. (2019). Research on Tissue Culture Rapid Propagation Technology of Taiwan Lily. Paper presented at the IOP Conference Series: Materials Science and Engineering. DOI: https://doi.org/10.1088/1757-899X/677/2/022014
Liu, Q., Yuan, L., & Liu, Q. (2011). Effects of Auxin and Cytokinin on Regeneration from Leaves and Scales of Lilium× formolongi'Raizen No. 1'In Vitro. Acta horticulturae, 900, 357-367. DOI: https://doi.org/10.17660/ActaHortic.2011.900.45
Novak, S. D., Luna, L. J., & Gamage, R. N. (2014). Role of auxin in orchid development. Plant signaling & behavior, 9(10), e972277. DOI: https://doi.org/10.4161/psb.32169
Pandey, R., Singh, A., & Mamta Sharma, M. S. (2009). In vitro propagation of Lilium.
Rafiq, S., Rather, Z., Bhat, R. A., Nazki, I., Al-Harbi, M. S., Banday, N., Ahmed, A. F. (2021). Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv.‘Ravenna’. Saudi journal of biological sciences, 28(12), 7581-7587. DOI: https://doi.org/10.1016/j.sjbs.2021.09.064
Sindhu, S., Chandanie, M., & Dhiman, M. (2016). Improved micro propagation protocol in Lilium hybrida var. Pollyanna.
Song, J.-y., Lee, Y.-y., Yi, J.-y., Lee, J.-r., & Yoon, M.-s. (2021). Enhancing in vitro Growth of Bulbs for Mass Propagation of Lily Germplasm. Korean Journal of Plant Resources, 34(1), 17-22.
Su Jiang, S. J., Cen ZhongYong, C. Z., Yang YanHua, Y. Y., Liang Shen, L. S., Wei JinLan, W. J., & Lu ZhaoCen, L. Z. (2014). Tissue culture and micropropagation of Lilium casa.
Taha, L. S., Sayed, S. S., Farahat, M., & El-Sayed, I. M. (2018). In vitro culture and bulblets induction of Asiatic hybrid lily'red alert'. Journal of Biological Sciences, 18(2), 84-91. DOI: https://doi.org/10.3923/jbs.2018.84.91
Tang, Y., Liu, X., Gituru, R. W., & Chen, L. (2010). Callus induction and plant regeneration from in vitro cultured leaves, petioles and scales of Lilium leucanthum (Baker) Baker. Biotechnology & Biotechnological Equipment, 24(4), 2071-2076. DOI: https://doi.org/10.2478/V10133-010-0077-4
Wang, W., Zhao, X., Zhuang, G., Wang, S., & Chen, F. (2008). Simple hormonal regulation of somatic embryogenesis and/or shoot organogenesis in caryopsis cultures of Pogonatherum paniceum (Poaceae). Plant cell, tissue and organ culture, 95, 57-67. DOI: https://doi.org/10.1007/s11240-008-9414-9
Youssef, N. M., Shaaban, S. A., Ghareeb, Z. F., & Taha, L. S. (2019). In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv.“Starfighter” plants. Bulletin of the National Research Centre, 43, 1-9. DOI: https://doi.org/10.1186/s42269-019-0246-z
Zeng, F., Zhang, X., Jin, S., Cheng, L., Liang, S., Hu, L., . . . Cao, J. (2007). Chromatin reorganization and endogenous auxin/cytokinin dynamic activity during somatic embryogenesis of cultured cotton cell. Plant cell, tissue and organ culture, 90, 63-70. DOI: https://doi.org/10.1007/s11240-007-9253-0
Zhao, Y. (2010). Auxin biosynthesis and its role in plant development. Annual review of plant biology, 61, 49-64. DOI: https://doi.org/10.1146/annurev-arplant-042809-112308
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Haider Ali, Iqbal Hussain, Kazim Ali, Saima Noor, Amber Imtiaz, Muhammad Zeeshan, Mumtaz Hussain, Sadia Sarwar
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a 