EFFECT OF PHYTOHORMONES ON SHOOT AND ROOT REGENERATION IN ROSE UNDER IN VITRO CONDITIONS

Abstract

n vitro propagation of rose has played a very important role in rapid multiplication of cultivars with
desirable traits and production of healthy and disease free plants. Murashige and Skoog medium supplemented with
various concentrations and combinations of Indole-3-butyric acid (IBA), Naphthylacetic acid (NAA),
Benzylaminopurine (BAP), and Indole-3- acetic acid (IAA) were employed for shoot regeneration in this study,
whereas MS½ and full strength medium containing different amounts of IBA (0.5 and 1mg/l) were used for root
induction. The highest multiple shoot formation was recorded in MS medium containing IAA (1 mg/l) and BAP (4
mg/l) with 30 g/l sugar. Cytokinin (BAP) in addition to auxin (IBA) caused the maximum bulbus formation which
turned into elongated shoots, while the NAA was observed to have antagonistic effect on shoot formation. Highest
numbers of roots were produced on MS½ strength + IBA 0.5 mg/l. The aim of this study was to determine growth
and development of rose on different growth media, to shorten the multiplication time of virus-free plant material.