EFFECT OF SUCROSE ON OPTIMAL ROOTING OF MUSA SPP. PLANTLETS IN-VITRO CONDITION

Abstract

To evaluate the effect of various concentrations of sucrose on in vitro rooting of regenerated banana micro
shoots. Three selected Musa varieties GCTCV-215 (AAA), Yangambi (AAA) and FHIA-23 (AAAA) were
cultured in half strength MS media supplemented with root promoting growth regulator 1.0 mg/L-1 indole
butyric acid (IBA) and different concentrations of sucrose (0, 20, 40, 60 and 80 g/L-1). Significant differences
(p≤ 0.05) were noticed in all Musa clones. Results indicated that as compared to control out of different
concentrations of sucrose all varieties, showed the maximum number of roots as well as highest root length in
MS medium augmented with 40 % and 20 % g/L sucrose respectively. Appropriate result among the varieties, in
terms of showing maximum roots was observed in GCTCV-215, followed by Yangambi. Effectual rooting on in
vitro is helpful for the successful establishment of these Musa varieties in field condition.