EFFECTS OF DIFFERENT PHYTOHORMONES ON SUGARCANE (SACCHARUM SPP.) REGENERATION

Abstract

Three sugarcane clones, AEC82-223, Ghulabi-95 and AEC86-328 were investigated
for callus induction and regeneration under different concentrations (2mg/L and 4mg/L) of
various auxins i.e. 2,4-dichlorophenoxyacetic acid (2,4-D), indol acetic acid (IAA), 3,6-
dichloro 2-methoxy benzoic acid (Dicamba) and picloram. An efficient callus induction was
observed on the medium containing picloram followed by 2,4-D but high callus proliferation
was achieved with picloram. Non-regenerable callus was produced when calli were
transferred for proliferation on Dicamba. The maximum calli induction, its proliferation and
plantlets regeneration were recorded in clone AEC82-223, while the minimum in AEC86-
328. The maximum chlorophyll mutation frequency was noted in clone AEC86-328 and
minimum in AEC82-223. Among auxin especially 2,4-D induces more genetic variability as
compared to other auxins used in this work.