PROTOCOL OPTIMIZATION FOR EFFECTIVE IN VITRO ROOT FORMATION OF SUGAR CANE SOMA CLONES IN NIA-2012, NIA-105 AND GULABI-95 VARIETIES

Abstract

The aim of this study is to develop an efficient In vitro regeneration and root formation procedure from
callus. Present work was conducted in 2015 at Nuclear Institute of Agriculture, Tando jam. Study relevant with
three sugarcane varieties of NIA like NIA-2012, NIA-105 and GULABI-95. Root formation of plantlets was
compared under different concentration of auxins IBA (1.0, 2.0, 2.5 mg1-1 and sucrose 2, 3 and 4 % respectively.
Highly significant (p<0.05) variations were observed for all parameters of root formation. Interactive effect of
variety treatment and concentration was non-significant for the number of root and length of root formation. Auxins
3% and sucrose 4% mgl-1 were highly effective for rooting. In vitro root formation for all genotypes of sugarcane
showed the maximum root and highest length in ½ MS media supplemented with 2.5 mg1-1 IBA and 4% sucrose,
therefore this concentration of growth regulators was used for future in vitro culture of sugarcane.