BIOPROCESS OPTIMIZATION FOR PECTINASE PRODUCTION IN A SUBMERGED CULTIVATION SYSTEM USING VARIOUS FILAMENTOUS FUNGI.

Ghulam Sughra Mangrio; Sippi Pirah; Nadia Mangrio; Asma Asghar; Bharat kumar; Altaf  Simair; Farooque Ali Bughio

doi:10.34016/pjbt.2023.20.02.812

Authors

Ghulam Sughra Mangrio Department of Biotechnology, Sindh Agricultural University, Tandojam 70060, Sindh
Sippi Pirah College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China
Nadia Mangrio Sugarcane Research Institute, Agriculture Research Centre, Tandojam, 70060, Sindh
Asma Asghar Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, 60800, Pakistan
Bharat kumar Department of Biotechnology, Sindh Agricultural University, Tandojam 70060, Sindh
Altaf Simair Department of Botany and Biotechnology GC University Hyderabad Sindh
Farooque Ali Bughio Department of Botany, Government College University, Hyderabad, 71000, Sindh

DOI:

https://doi.org/10.34016/pjbt.2023.20.02.812

Keywords:

Biosynthesis, Pectinase, Agro industrial waste, Filamentous fungi,, Date syrup Aspergillus fumigatus, Aspergillus niger, Mucor geophillus, submerged fermentation

Abstract

The pectinase enzymes attack in several ways; the pectin is widely used in processing fruit juices, extracting vegetable oil, processing alcoholic drinks, and various uses in food industries. The present study was carried out to utilize an agro-industrial by-product (date syrup) as a carbon source for the growth of different fungi and the production of the pectinase enzyme. Four types of fungi (Penicillium lilacinum, Mucor geophillus, Aspergillus niger and Aspergillus fumigatus) were used in this study. The maximum production rate of pectinase was observed by A. niger when grown on a mineral medium incorporated with 2.5% and 5% date syrup compared to other fungi used. The highest rate of pectinase, 5.68 units/ml, was produced after 72 hours when A. niger was grown on 5% date syrup.

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