MOLECULAR IDENTIFICATION OF BACTERIAL SPECIES IN STREET VENDED CHOLAY CHAT USING 16S RRNA GENE
DOI:
https://doi.org/10.34016/pjbt.2023.20.02.800Keywords:
Street vended Cholay Chat, molecular identification, 16S rRNA geneAbstract
The contamination of pathogenic microbes in street vended foods is a serious public health concern associated
with foodborne illnesses (FBIs). The street vended Cholay Chat (SVCC) is a popular savory chickpea-based
ready-to-eat food in Pakistan. The microbial quality of 18 SVCC samples was assessed through conventional and
molecular identification. A total of three (03) bacterial species (i.e., Bacillus cereus, Macrococcus caseolyticus
and Staphylococcus sciuri) were characterized via phenotypic and biochemical analysis in SVCC. Among them,
B. cereus remained highly prevalent (88.88%) followed by M. caseolyticus (61.11%) and S. sciuri (11.11%). The
polymerase chain reaction (PCR) based DNA amplification of the 16S rRNA gene and sequencing of isolated
bacterial species were performed. The BLASTn analysis revealed that sequences were significantly aligned with
B. cereus (97.38 %), M. caseolyticus (93.16%), and S. sciuri (97.21%). It is concluded that the microbial quality
of SVCC is highly detrimental and thus is liable to cause FBIs among consumers. Therefore, safe food handling
and manufacturing practices must be mandatorily assured by street food vendors to lessen the burden of FBIs
among consumers
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