ESTIMATION OF MINIMUM INHIBITORY CONCENTRATION OF ANTIBIOTICS USING MICRO-TITER PLATE METHOD FOR NATIVE MULTI DRUG RESISTANT GRAM NEGATIVE BACTERIA

Authors

  • Kanwal Ibrahim Department of Microbiology, University of Karachi, Karachi-75270, Pakistan
  • Asma Saeed Department of Microbiology, University of Karachi, Karachi-75270, Pakistan
  • Sadia Khalil Federal Urdu University of Arts Science and Technology, Karachi, Pakistan
  • Amna Alam Department of Microbiology, University of Karachi, Karachi-75270, Pakistan

DOI:

https://doi.org/10.34016/pjbt.2023.20.01.773

Keywords:

gram-negative, resistant, MDR, clinical, antibiotics

Abstract

Gram-negative bacteria were collected from different clinical labs situated in Karachi. Of these 83 isolates, 99% were found resistant against one or more of the following antibiotics: ampicillin, co-amoxiclav, gentamycin, neomycin, streptomycin and tetracycline. Most of the isolates were multi-drug resistant (MDR) having resistance to three or more antibiotics at a time. For ampicillin, 4.8% of the isolates were found Susceptible, 3.6% were Intermediate while 91.5% were found Resistant. For co-amoxiclav, 1.2% of the isolates were found Susceptible, 6% were Intermediate while 92.7% were found Resistant. For gentamicin, 3.6% of the isolates were found Susceptible, 10.8% were Intermediate while 86.7% were found Resistant.  For neomycin, 21.6% of the isolates were found Susceptible, 40.9% were Intermediate while 43.4% were found Resistant. For streptomycin, 1.2% of the isolates were found Susceptible, 18% were Intermediate while 91.6% were found Resistant.  For tetracycline, 15.7% of the isolates were found Susceptible, 45.7% were Intermediate while 53% were found Resistant. The study confirmed the presence of multidrug resistance in indigenous clinical gram-negative bacteria which is an alarming situation and needs development of effective alternative strategies for the treatment of infections.

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Published

2023-04-13

How to Cite

Ibrahim, K., Saeed, A., Khalil, S., & Alam, A. (2023). ESTIMATION OF MINIMUM INHIBITORY CONCENTRATION OF ANTIBIOTICS USING MICRO-TITER PLATE METHOD FOR NATIVE MULTI DRUG RESISTANT GRAM NEGATIVE BACTERIA. Pakistan Journal of Biotechnology, 20(01), 1–9. https://doi.org/10.34016/pjbt.2023.20.01.773

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Research Articles