PREVALENCE OF STAPHYLOCOCCUS AUREUS AMONG GINGIVITIS IN PATIENT WITH ORTHODONTIC WIRES IN KUFA CITY /IRAQ.

Abstract

This study aimed to investigate the role of orthodontic wire on bacterial community in oral cavity as well as its effectiveness on Staphylococcus aureus. A total of 78 gingival swab samples have been collected from patient with orthodontic wires suffering from gingivitis and 71 samples were collected from healthy without orthodontic wire during four months from private dental clinics. The results of conventional isolation and identification of bacterial isolates as well as biochemical test showed that S. aureus was the commonest causative pathogens among acute gingivitis (46%) followed by Granulicatella adiacens (29.6%) where 36 isolates were isolated from acute and chronic gingivitis in comparison with 27 isolates from healthy. A mutation experiment was carried out to explain the role of orthodontic wires on S. aureus. The results revealed the same change has been obtained when using both of Nikle-titanium (NiTi) and stainless steel wire on S. aureus after 24-96 hr from incubation. Antibiotic sensitivity pattern of both original and treated isolates with NiTi and stainless steel wires explained increased in antibiotic resistance of S. aureus to bacitracin, ceftazidim, ogmentin, and erythromycin while other antibiotic remain sensitive such as cefotaxim and amikacin.