GENETIC ENGINEERING OF POTATO PLANT (Solanum tuberosum L.) cv. JALA IPAM WITH MmPMA GENE ENCODING PLASMA MEMBRANE H+-ATPASE

Abstract

Plasma Membrane (PM) H+-ATPase has important function as primary transporter driving secondary active transport systems in plant. Some plants overexpressing PM H+-ATPase showed increased light-induced open stomata number and plant growth. This study has an objective to overexpress PM H+-ATPase expected can increase productivity of potato. Genetic transformation was done by cocultivation method using Agrobacterium tumefaciens strain LBA4404 carrying pGWB502-MmPMA. Efficiency of transformation in this study is 3.46%. Molecular analysis with PCR was carried out by using primer 35S-F and PMA-R1 to ensure integration of MmPMA into Jala Ipam potato. PCR analysis showed that all of six putative transgenic Jala Ipam potato lines are containing MmPMA gene under the control of 35S CaMV. This study showed that genetic transformation process in Jala Ipam potato with MmPMA gene was successful with enhancing light-induced open stomata percentage, open stomata pore width, and root elongation-under low pH condition