REGENERATION OF PLANTS IN EMS TREATED LOCAL MUNG BEAN (VIGNA RADIATE L. WILCZEK) UNDER SALT STRESS

Abstract

The regeneration of EMS treated seeds of two local varieties of mung bean was investigated
under salt stress conditions. Three explants e.i. cotyledon, leaf and shoot apical meristem of mung
bean obtained from in vitro grown EMS treated seeds of varieties NM-92 and Khalood were
inoculated on MS medium containing various concentrations of 2, 4-D and BAP and IBA. The
highest callus proliferation (98.3%) was obtained in EMS treated leaf of NM-92 followed by 96.5%
in shoots of NM-92 control on MS medium supplemented with 1.8μM 2, 4-D and 3.56μM BAP.
The increase in callus fresh weight was higher in leaf explants of both treated varieties. Similarly
57.3±1.3 callus cultures of treated leaf explants of NM-92 were survived on media containing
50mM NaCl. Similarly the highest regeneration (65.5±2.1%) was observed in NaCl selected callus
cultures raised from shoots of EMS treated seeds.