COMPARISON OF DIFFERENT DOSES OF PLANT GROWTH HORMONES ON CALLUS INDUCTION AND REGENERATION IN SUGARCANE

Authors

  • Simair A. Altaf, Mangrio G. Sughra, Wain M. Shahbaz, Thebo K. Nasreen, Mangrio Sher M. and Dahot M. Umar Department of Biotechnology, Sindh Agriculture University, Tandojam, Sindh, Pakistan.

Abstract

Sheath rolls of three sugarcane cultivars (BL-4, Gulabi-95 and NIA-2004) were grown
for callus induction and subsequent in vitro plant regeneration on basic medium
containing Murashige and Skoog (MS) supplementd with different concentrations of 2, 4-
D for callus induction. Media comprised of MS+BAP and MS+NAA+Kin with various
concentrations for shoot induction and root induction respectively. Maximum callus was
obtained with MS+5.0 mg/L 2,4-D. Shoot induction was best at MS+2.0 mg/L BAP and
best root induction was observed at MS+NAA at 2.0 mg/l + 1.25 mg/L Kinetin.

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Published

2009-07-26

How to Cite

Simair A. Altaf, Mangrio G. Sughra, Wain M. Shahbaz, Thebo K. Nasreen, Mangrio Sher M. and Dahot M. Umar. (2009). COMPARISON OF DIFFERENT DOSES OF PLANT GROWTH HORMONES ON CALLUS INDUCTION AND REGENERATION IN SUGARCANE. Pakistan Journal of Biotechnology, 10(1), 27–30. Retrieved from https://pjbt.org/index.php/pjbt/article/view/611

Issue

Vol. 10 No. 1 (2013): Pak. J. Biotechnology

Section

Research Articles

License

Copyright (c) 2021 Simair A. Altaf, Mangrio G. Sughra, Wain M. Shahbaz, Thebo K. Nasreen, Mangrio Sher M. and Dahot M. Umar

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.