PHYLOGENETIC IDENTIFICATION OF BACTERIA WITH ANTIMICROBIAL ACTIVITIES OF TUNICATE Ascidia ornata FROM DORERI BAY

Abstract

We investigated the interaction between tunicate Ascidia ornata and microorganism. This microorganism can be interpreted as a source of food or symbionts for mutualism. Symbiosis of microorganism with tunicate may produce metabolites that have biological activity like antimicrobial. Ascidia ornate were collected from hard coral at Lemon Island Doreri Bay Manokwari. Bacteria isolate of tunicate Ascidia ornatashowed antimicrobial activities against Gram-negative, Gram-positive and the fungus Candida albicans. Eight isolates which can have antimicrobial activity like Bacillus cereus, Bacillus sp, Bacillus megaterium, Bacillus pumilus, Enterobacter sp, Enterobacter hormaechei and Ochrobactrum sp. The isolate was identified with 16S rDNA sequencing with 99 – 100 % sequence similarities. Bacillus species was identified can against all human pathogenic human isolates including methicillin-resistent Staphyloccocus aureus. The marine symbionts bacteria collected has potential to inhibit human pathogenic microbes and could be used as raw material for medicine.