EFFECT OF SOLVENT IN COMBINATION WITH ULTRASONIC WAVE ASSISTANCE ON TRITERPENOID EXTRACTION FROM RED LINGZHI MUSHROOM (Ganodermalucidum)

Abstract

Triterpenoid is one of the compounds that contribute tored lingzhi mushroom's major significant therapeutic activities (G. lucidum). Triterpenoids have anti-allergic, anti-cancer, anti-tumor, and anti-aging effects, enhance oxygen utilization, free radical scavenging, lowerblood pressure, inhibit cholesterol biosynthesis, and enhance liver function and blood pressure, inhibit cholesterol biosynthesis, and improve liver functionetc. The beneficial effects of triterpenoids in Ganoderma lucidum on human health have generated interest in developing methods for extracting them from the fruit bodies of G. lucidum. This article presents the effect of solvent in combination with ultrasonic wave assistance in triterpenoid extraction ability from red lingzhi mushroom. Before carrying out triterpenoid extraction; Red lingzhi mushroom was ground 2mm in size. Four parameters of the extraction process, including the ethanol concentration, ratio (v:w) between ethanol solvent and mushroom, extraction time, , extraction temperature, ultrasonic time and ultrasonic intensity were determined. The extraction without ultrasonic waves for 180 minutes was used as a control. The total triterpenoid content was collected during the extraction process. The results showed that red lingzhi mushroom was extracted by ethanol solvent at a concentration of 85%, the ratio between ethanol solvent and mushroom was 3:1 (v:w), ultrasound intensity was 58 W/cm2, frequency was 20 kHz), extracting temperature was 55°C, removing time was 5 min had total triterpenoid content 3.06 times higher than the control.