STUDY THE EFFECT OF CLADOPHORA GLOMERATE ALGAE EXTRACT ON THE PARASITE OF ENTAMOEBA HISTOLYTICA

Abstract

Was isolated parasitic amoeba fabric E.histolytica in samples bloody diarrhea mucosa of patients infected with Wales a hot amoebic Amoebic dysentery and has its own development in the center of Luc-eggs (LE) axis to add protein pro complex) GALNER) instead of white emulsion, the results showed the success of the implant in the middle, accompanied bacteria, growth continued for two weeks, included this term secondary farm to perpetuate the amoeba. The effectiveness of selected two concentrations chloroform extract cladophora glomerat first concentration 128 mg / ml and second concentration 256 mg / ml. The results showed the effect of two concentrations against amoeba condition of the fabric in the glass. the effectiveness of the extract with the increase mixture focus, where it came from homicide rate of 80% when using the extract 128 mg / ml and 90% when using concentration 256 mg / ml, has influence came to extract a higher content of alkaloids, phenols and Alsabonyat and Alkleikosadat. In-vitro was, VFD was measuring enzyme AIP in the positive control 0.28± 28.6 K.A.U / 100ml and dosed Group by concentration 128 mg / ml was 11.48± 0.6 K.A.U/ 100ml and7.42± 0.055 K.A.U/ 100ml in the second concentration as compared with the negative control group5.23± 0.072 K.A.U/ 100ml treated falagel 7.21± 0.080 K.A.U/ 100ml. enzyme LDH results were in the range of positive control 68.82± 0.3425 U/ L dosed group by concentration 128mg/ ml was50.28± 0.1398 U/ L. As the second concentration 36.36± 0.3425 U/ L. As compared to negative control Group25.28± 0.3425 U/ L group dosed by flagel 51.11± 0.16 U/ L. The cholesterol was in the positive control group and the group141.3± 10.1 U/ L dosed group 128mg/ ml was 156± 0.3422.9 U/ L the second concentration 174.17± 4.40 U/ L as compared to the negative control group184.53± 0.26 U/ L by filagel treatment group 180.5.± 0.221 U/ L, Keriaten was within the normal boundaries (1.2-0.5) mg/dl. A concentration of glucose in the positive control group was 92.28± 5.55 U/ L and the dosed group by low concentration was 86.6± 0.34211 U/ L. The high concentration was 95.5± 5.11 U/ L compared to the negative control group 99.32± 3.41 U/ L and the treatment group95.5± 5.11 U/ L. The total protein concentration, was positive in the control group 6.65± 0.33 U/ L and the dosed by the low first concentration 7.71± 2.84 U/ L and the higher 6.53± 0.561 U/ L as compared with the negative control control 7.21± 0.802 U/ L and treatment by filagel 6.25± 0.31 U/ L.