EFFECT OF GROWTH REGULATORS AND SUCROSE ON THE INDUCTION AND PRODUCTION OF FLAVONOIDS IN CALLUS OF VITIS VINIFERA (L) IN VITRO

Abstract

The study included the use of plant tissue culture technique in the induction of the callus of Vitis vinifera and to stimulate the production of flavonoids. The study was carried out in two stages after sterilization: The first stage was the establishment of callus by culturing a single-node on the MS medium which contained 2,4-D and IAA at different concentrations of 0, 1, 1.5, 2, 2.5mg/l with a fixed concentration of 0.5mg/L of BA in independent experiments. The second stage was implemented by cultivating the callus that was induced in the first stage on the MS medium which was provided with different concentrations of sucrose of 30, 60, 90, 120g/l. Results of the study showed that the auxin 2,4-D achieved the highest wet and dry callus weight of 1817.20 and 94.38mg respectively, while the IAA achieved a wet and dry callus weight of 660.07 and 66.43mg respectively. The MS medium that was provided with 2.5mg/l auxin achieved the highest wet and dry callus weight of 977.77 and 122.35mg respectively. The 2,4-D at the concentration of 2mg/l gave significantly higher wet and dry callus weight of 2722.50 and 146.50mg respectively compared with other treatments.