EFFECT OF ZIZIPHUS SPINA CHRISTY EXTRACT IN BIOFILM FORMATION OF METHICILLIN RESISTANCE STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCUS HAEMOLYTICUS

Abstract

This research aimed to investigate the antibacterial activity of hot an aqueous extract of leaves Ziziphus spina Christy (Sidr), against biofilm formation of clinical isolates Staphylococcus aureus and Staphylococcus haemolyticus. Ten isolates were diagnosed initially as Staphylococci then selected four isolates depending on virulence and resistance to different types of antibiotics. After that VITEK-2compact system (ID and AST) was used to confirm the species of Staphylococci. The results showed that three isolates reverting to Staphylococcus aureus and one to Staphylococcus haemolyticus. Investigation of S. aureus and S. haemolyticus isolates ability to forming biofilm using of Microtiter plate (96 well) methods, the results indicated that all of the isolates were able to produce the biofilm. The effect of Moxifloxacin and Penicillin G with (MIC), (Sub-MIC) and (Sub-Sub-MIC) were detected for preventing of S.aureus and S.haemolyticus biofilm production, as well as hot an aqueous extract of leaves Ziziphus spina Christy (Sidr) with50 mg/ml tested against the biofilm formation, the results showed ability of tow antibiotics and plant extract to prevent biofilm formation.