MOLECULAR DETECTION OF ANTIBIOTIC RESISTANCE GENES OF ACENITOBACTER BUMANNII

Abstract

This study aims to detection antibiotic resistance gene in Acenitobacter bumannii isolates collected from different diseases. It was included 276 specimens collected from different sources including patient suffer from burn, wound, upper respiratory tract inflammation, urinary tract inflammation, osteocytes, endocarditis and blood infection. 52 of these isolates belonged to italic pleas which have been identified through several laboratory tests including the morphology characterizes, biochemical, API 20 E and VITK -2 compact system technique. The distribution of isolates was burn (34.6 %), wound (28.8 %), urine (19.2), blood (9.6%), sputum (5.7%), plural fluid and CSF (1.9 %). The molecular study revealed that A. baumannii have the gene of ESBL, oxacillance and MBLs bla OXA like 51, bla OXA like 24, bla PER, bla TEM is (94%, 89%, 84%, 96%) respectively also the gene aadA and aadB is (76% and 84%) respectively fluroquinolone gyrA (66%) and finally all isolates have the gene bla TEM.