INCIDENCE OF A BRIGHTER VARIANT OF VIBRIO HARVEYI STRAIN N2 UNDER CONTINUOUS LAB CULTURING

Abstract

A brighter variant of a lab cultured strain of Vibrio harveyi was observed under routine lab culturing procedures and was investigated for differences in luminescence characteristics and protein profile as compared to the wild type parent. A lysate of the variant and wild type strain was subjected to BCA protein analysis and proteins were separated on PAGE to elucidate differences. Luminescence studies revealed a faster growing, 20% brighter variant that had a clear difference in protein profiles when subjected to quantitative and qualitative protein analyses. The bright variant of N2 had 0.21µgm/µl more total protein content as compared to the wild type and exhibited darker protein bands for some of the proteins separated on PAGE.