OPTIMIZATION OF CULTURE CONDITIONS TO PRODUCE SECONDARY METABOLITES BY PLEUROTUS OSTREATUS

Abstract

The specie of mushroom Pleurotus ostreatus grown on the Potato Dextrose Agar (PDA) medium for the activation and then inoculated in the fermentation medium to obtain maximum production of metabolite. Maximum production of metabolites produced by Pleurotus ostreatus at different incubation time periods. The effect of different carbon sources glucose, galactose, fructose, sucrose and starch was checked on the growth and production of metabolite by Pleurotus ostreatus and the higher production was achieved with glucose. The effect of different nitrogen sources like potassium nitrate, sodium nitrate, ammonium sulphate, casein and urea were checked on the growth and production of metabolites by Pleurotus ostreatus. Among the different nitrogen sources maximum metabolite were obtained with casein. The effect of pH on the growth and production of metabolites by Pleurotus ostreatus was studied in the range of 4.5-8.5 and maximum production was noted with 4.5 pH. The effect of temperature was checked on the growth and production of metabolite by Pleurotus ostreatus in range of 25 ̊C-35 ̊C and higher production of metabolites were achieved at 30 ̊C.