XYLANASE PRODUCTION FROM BACILLUS SUBTILIS IN SUBMERGED FERMENTATION USING BOX-BEHNKEN DESIGN

Abstract

In this study, an attempt was made to optimize the nutritional conditions for xylanase production by Bacillus subtilis in submerge fermentation process using agricultural waste like corn cobs as substrate. Three variables with three levels such as corn cobs loading (1, 3, 5, % w/v), peptone (0.05, 0.275, 0.5%) and KH2PO4 (0.1, 0.3, 0.5%) were optimized through Box-Bhenken design of response surface methodology. It was revealed that the maximum yield of xylanase (295 U/ml) was achieved with 3% corn cobs as substrate, 0.05 (%) peptone, and 0.5 (%) KH2PO4. Analysis of variance reveals that the proposed model was significant having an F value of 188.77 and its corresponding p values 0.000 and, P>F<0.0001 shows the model’s accuracy. Higher R2 values (98.93) of the model depicted that only 1.07% variations could not be explained by the model. Findings of this study could be utilized for industrial exploitation of the enzyme.