HYPEROSMOTIC STRESS TOLERANCE OF TRANSCRIPTION ACTIVATOR Msn2- OVEREXPRESSION STRAIN AND PROLINE-NO SYNTHESIS STRAIN OF Saccharomyces cerevisiae IN VERY HIGH GRAVITY BIOETHANOL FERMENTATION

Authors

  • Dwi Aryanti Nur’utami Major Program of Biotechnology, Graduate School of Bogor Agricultural University, Indonesia
  • Liesbetini Haditjaroko Department of Agroindustrial Technology, Faculty of Agricultural Technology, Bogor Agricultural University, Indonesia
  • Hiroshi Takagi Laboratory of Applied Stress Microbiology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan
  • Khaswar Syamsu Department of Agroindustrial Technology, Faculty of Agricultural Technology, Bogor Agricultural University, Indonesia

Keywords:

bioethanol, hyperosmotic stress, very high gravity fermentation

Abstract

The aim of this study was to assess the tolerance of two modified commercial Saccharomyces cerevisiae Ethanol Red, Pro1(I150T)/Mpr1(K63R) and MSN2-OP in hyperosmotic stress and to measure the kinetic parameters of the best yeast from both of them in the production of bioethanol in very high gravity ethanol fermentation. The results show that Pro1(I150T)/Mpr1(K63R) yeast strain is more tolerant to hyperosmotic stress than MSN2-OP. The Pro1(I150T)/Mpr1(K63R) yeast strain is tolerant up to 70% sucrose and 13% ethanol, while the MSN2-OP yeast strain has been inhibited by 70% sucrose and 11% ethanol. The Pro1(I150T)/Mpr1(K63R) yeast strain produces about 14.6 ± 0.3% (w/w) ethanol for 48 h fermentation with final residual sugar about 9.43% and 83.9 ± 0.6% substrate consumption efficiency.

Metrics

Metrics Loading ...

Downloads

Published

2017-06-25

How to Cite

Nur’utami, D. A. ., Liesbetini Haditjaroko, Hiroshi Takagi, & Khaswar Syamsu. (2017). HYPEROSMOTIC STRESS TOLERANCE OF TRANSCRIPTION ACTIVATOR Msn2- OVEREXPRESSION STRAIN AND PROLINE-NO SYNTHESIS STRAIN OF Saccharomyces cerevisiae IN VERY HIGH GRAVITY BIOETHANOL FERMENTATION . Pakistan Journal of Biotechnology, 14(2), 135 – 139 . Retrieved from https://pjbt.org/index.php/pjbt/article/view/468

Issue

Vol. 14 No. 2 (2017): Pak. j. Biotechnology.

Section

Research Articles