OPTIMIZATION OF PEDIOCIN N6 PRODUCTION BY ISOLATE Pediococcus pentosaceus STRAIN N6 WITH MODIFICATION OF CARBON AND NITROGEN SOURCES

Abstract

Pediocin bacteriocin was produced by isolates Pediococcus pentosaceus strain N6. This Pediococcus pentosaceus strain N6 was isolated from Hot Springs RimboPanti West Sumatra. The aim research was to optimize the production Pediocin N6 using modified of carbon and nitrogen sources. Moreover, the testing temperature and pH optimum to obtain the highest antimicrobial activity against pathogens Escherichia coli O157:H7, Salmonella thyphimurium and Listeria monocytogenes as well as optimum time in producing Pediocin N6. The growth of cells isolate and optimum production of Pediocin N6 occurs at the end phases logarithms that is between 19-20 hours until the end of the exponential phase is between 35-36 hrs. Antimicrobial Activity of isolated Pediococcus pentosaceus strain N6 was reached optimum 50oC with diameter inhibition zone of 29.6 mm against the bacteria Listeria monocytogenes, 20.3 mm against the E. coli O157: H7 and 18.4 mm against the S. thyphimurium. The optimum pH of antimicrobial activity is pH 5 with diameter inhibition zone of 29.7 mm against the bacteria Listeria monocytogenes, 21.4 mm forthe bacterium E. coli O157: H7 and 19.3 mm forthe bacterium S. thyphimurium. Incorporation of molasses as a carbon source to produce diameter inhibition zone of 34.5 mm against the bacterium L. monocytogenes, 27.9 mm against E. coli O157: H7 and 26.2 mm against S. thyphimurium and skim milk as nitrogen sources to produce diameter inhibition zoneof 3.7 mm against the bacterium L. monocytogenes, 27.6 mm against E. coli O157: H7 and 26.8 mm against S. thyphimurium. Modification of molasses as a carbon source and skim milk the nitrogen source produces highest antimicrobial activity.