HEMATOLOGICAL EFFECTS OF GRAPHENE NANOPARTICLES EXPOSED TO COMMON CARP CYPRINUS CARPIO L.

Abstract

The current was research conducted to study the hematological effects of graphene nanoparticles exposed to common carp Cyprinus carpio L. A total of 48 specimens of Cyprinus carpio with average of 68.44 g in weight were examined to detect the expose graphene nanoparticles on experimental fish. Experimental fish were acclimated for two weeks prior to initiation of the experiment to adapt to laboratory conditions. Fish were exposed to graphene nanoparticles as followed T1, 10; T2, 20 mg / l and control group (without graphene) for 10 days. The study included measuring the hematological parameters of the experimental fish including RBC, WBC, PCV and Hb. Results of statistical analysis showed significant decrement at P ≤ 0.05 for both RBC (0.770 Cells / μlx106) in T1 and 0.850 Cells / μlx106 in T2 compared with negative control (1.410 Cells / μlx106). No significant decrease P > 0.05 of each PCV in T1 and T2 (31 and 27%) respectively compared with control negative (35%) and hemoglobin (10.30 and 9 mg/dl) in T1 and T2 respectively compared with negative control (11.65 mg /dl), while the addition of graphene nanoparticles did not effect on the number of WBC in T1 and T2 (15.1 and 19.1 Cells / μlx103)) respectively compared with negative control (15.6 Cells / μl x103). The present study was suggested to investigate of the graphene nanoparticles effects to clarify a clear representation about on fish and its danger of its accumulation in aquatic environment.