QUALITATIVE DETECTION OF HIGH RISK HPV AND GENOTYPING ASSOCIATED CERVICAL ABNORMALITIES

Abstract

Introduction: Change in cervix epithelial tissue as abnormal cells as dyskaryosis or dysplasia can be due to inflammation or infection. Human papilloma virus (HPV) is the main viral infection of the female reproductive system. The current study is aimed to detect the HPV genotypes that associate with cervical abnormalities.
Materials and methods: Ninety subjects included 70 Iraqi patients with cervical abnormalities and 20 apparently healthy which were included in the work. Pap smears were taken from the cervix of the subjects, DNA was extracted, and HPV genotyping was done to all samples.
Results: In general, our results showed that 42.86% of women had positive HPV infection and 57.14% negative HPV. The seventy cervical intraepithelial neoplasia biopsy that were obtained from patients classified histopathologically as: squamous cell Atypia 30 out of 70 (33.33 %), low grade squamous intraepithelial lesions (CIN I) constituted 18 out of 70 (20.00%) where as the high grade (CINII &III) constituted 16 out of total 70 (11.11% and 6.67 %) respectively and squamous cell carcinoma 6 out of total 70 (6.67 %). According to cytology patients samples were classified as: 10 (11.11%) with non-specific cervicitis, 18 (20.00%) cases of LSIL, 16(17.78%) cases of HSIL, 20 (22.22%) cases of ASCUS and squamous cells Carcinoma 6 (6.67 %).
Conclusion: The results showed that 30 of patients were positive to HPV. The common HPV genotype was HPV-16, HPV-59 and HPV-56 followed by HPV types 66, 51, 58 and 68. Also multiple infections of viruses were observed in patients. It was found that variation in the results showed high significant on each histological grads examination.