CORRELATION BETWEEN BIOFILM FORMATION AND BACTERIOCIN PRODUCTION BY LACTOBACILLUS ACIDOPHILUS

Abstract

The present study was carried out to investigate the Correlation between biofilm formation and bacteriocin production by Lactobacillus acidophilus A total of 214 vaginal swab of healthy women without vaginitis and/or urinary tract infection were collected from hospitals in Baghdad city .Vaginal swabs of each women inoculated in MRS broth medium, after 24 h incubation in the presence of 5% CO2 the specimens were sub-cultured on MRS agar and 49 samples from 104 samples were Lactobacillus acidophilus, while negative growth were 110 samples. The first identification presented then using conventional polymerase chain reaction (PCR) with specific primers gene which showed that 11 isolates were Lactobacillus acidophilus carried bacteriocin gene also 11 L. acidophilus isolates characterized by their ability to inhibit the growth of Staphylococcus aurous , Pseudomonas aeroginosa , Escherichia coli through the production of bacteriocin and all Lactobacillus acidophilus isolates form biofilm at different levels (weak, moderate and strong) were produced bacteriocin. This study suggested highly significant difference and strong correlation (r= 0.914; P = 0.00; P<0.01) between biofilm formation and bacteriocin production.