BIOTECHNOLOGICAL TECHNIQUES STIMULATE THE PRODUCTION OF SUGARCANE AND USEFUL FOR ENHANCING OF PHYSIOLOGICAL TRAITS

Abstract

An experiment was designed to develop technological method through which formulate protocol for tissue culture in sugarcane. Shoot tips of Saccharum officinarum L. as explants of three varieties included NIA-2012, NIA-105 and Gulabi-95. The regeneration of regenrable type of callus (whitened and solid type) was using in a basic medium with ABK (Indol acetic acid) ( indol butric acid) cytokinin (kintin) added with different content of auxin (2, 4-D), (NAA) and picloram. Main analysis of variance was showing that all the traits are significant in NIA-2012 which fallowed by NIA-105. The best combination for the multiplication and shoot rising of sugarcane the plant growth hormone was used 2, 4-D and NAA. Root proliferated with the NAA at the concentrations of 3.0 mg/L was observed. However, the optimized protocol can be used for rapid in vitro mass multiplication of three sugarcane varieties related to the field parameter enhance production of sugarcane hence minimize the limitations sugarcane planting materials.