ESTIMATION OF ATPASE ACTIVITIES AND EXTRACELLULAR ION CONCENTRATIONS OF (NA+, K+, CL-AND CA+2) AFTER IRRADIATION OF BLOOD SAMPLES WITH LASER 532NM AND LASER 650NM

Abstract

The present study was conducted to explain how light energy of laser can be affected of ion pumps (ATPase) and concentrations of available ions across cellular membranes.
A whole blood samples were collected and divided into three groups, the first group was remained without irradiation to serve as a control group and to perform comparisons between irradiated and non-irradiated samples, The second group was subdivided into two subgroups. The first subgroup was irradiated with laser 532nm, 4mw for 10 minutes and the second subgroup was irradiated with 650nm, 135mw for 10 minutes , there after these samples were used to determine the activities of ATPase and the obtained results showed a remarkable increase in the ATPase activities of irradiated blood samples when compared with non-irradiated blood samples .The results showed increased activities of ATPase in laser 650nm via increase concentrations of in organic phosphate (pi) resulted from hydrolysis of ATP to ADP.
Three group of samples was also subdivided into two subgroups. The first subgroup was consequently irradiated with laser 532nm, 4mw for 5 and 10 minutes. The second subgroups was also consequently irradiated with laser 650nm, 4mw for 5 and 10 minutes. After irradiation, the samples were used to determine the common available ions (Na+, K+, Cl- and Ca+2). Results of Na+ showed a progressive decrease in both irradiated blood sample (532nm, 650nm) when compared with non-irradiated samples. On the other hand, results of the other ions (K+, Cl-, Ca+2) showed a remarkable increase in all irradiated of blood- samples in a comparison with control group. The low levels of Na+ were found to be associated inversely with increase wave length of laser especially at 650nm.
On the other hand, the decrease in concentration of ion (Ca+, K+, Cl-) were directly proportional with wave length of laser beam.
In conclusion, the data obtained from this study indicated that light energy can be exert photo-biostimulation, in particular, on ATPases enzymes and increases their activities through increase the final product of inorganic phosphate (pi). At the same time, the laser energy can be affect ion channels responsible for distribution of ions across cell membranes and enhances the movement of ions across cellular membranes too much when blood samples become irradiated with laser energy.
The aim of this study involved to determine the effects of two types of laser (532nm and 650nm) with 4mw and 135mw on adenosine triphosphatase enzymes (ATPases) activities and then after estimation of some available ions to explain the effects of light energy on membrane potential (ion distributions).