GENOTYPING OF VAGINOLYSIN GENE OF GARDNERELLA VAGINALIS ISOLATED FROM PRETERM LABOR PATIENTS IN HILLA CITY

Authors

  • Ilham A. Bunyan, Lamees A. Abdul-Lateef, Azal Alaa Al-Rubaeaee Babylon university, college of medicine, Department of microbiology, Iraq.

Keywords:

G.vaginalis , cpn60 , vaginolysin, PCR-RFLPs, preterm labor

Abstract

The present study aims to detection Genotyping of vaginolysin gene for Gardnerella vaginalis which isolated from preterm labor patients by PCR-RFLP in Hilla cityIn this study, 150 clinical sample were collected from patients with preterm labor submitted to Al-Hilla Teaching Hospital and Babylon Maternity and pediatric hospital. After extraction of DNA from high vaginal swabs, the DNA was subjected to PCR- RFLP method for identification of G.vginalis. results show It was found only (20%) of bacteria was give positive result. However, vagainolysin were carried out using specific primer and the result shows that 12 samples were positive amplification 1551bp then products were digested with EcoR I the results showed that vagainolysin exhibited three fragment 1000, 717, 100 bp respectively, assertion that G.vaginalis have four Genotyping this improving that it is had more virulent

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Published

2018-06-25

How to Cite

Ilham A. Bunyan, Lamees A. Abdul-Lateef, Azal Alaa Al-Rubaeaee. (2018). GENOTYPING OF VAGINOLYSIN GENE OF GARDNERELLA VAGINALIS ISOLATED FROM PRETERM LABOR PATIENTS IN HILLA CITY. Pakistan Journal of Biotechnology, 15(2), 377–381. Retrieved from https://pjbt.org/index.php/pjbt/article/view/406

Issue

Vol. 15 No. 2 (2018): Pak. J. Biotechnology

Section

Research Articles