ANTAGONISTIC ACTIVITY OF SILVER NANOPARTICLES SYNTHESIS BY FUSARIUM OXYSPORUM AGAINST CANDIDA SPP.

Abstract

Background and Objective: Candida infections are one of the most commonly occurring fungal infections in human. Silver nanoparticles (Ag-NPs) are a new kind of material with several applications, such as antimicrobial against bacteria, fungi and viruses. The current education remained achieved to appraise the extracellular synthesis of silver nanoparticles by Fusarium oxysporum against Candida spp.
Methods: Ag-NPs synthesis from F.oxysporum extracellularly and characterized by UV-Visible spectral analysis, X-ray Diffraction Analysis (X-RD), Atomic force microscopy(AFM) and Fourier Transform Infrare Spectroscopy (FTIR),effect of Ag-NPs at concentrations (10, 25, 50,100μg/ml) against 7 isolates of Candida spp. by 2 way: agar plate well diffusion assay and Cell Proliferation Assay (MTT).
Results: Ag-NPs shown extreme absorbance peak on 418nm in UV–Visible Spectral. Since the X-RD design showed the presence three peaks, Strong diffraction peaks were: 38.19 º (111), 48° (200) and 67° (220). The Ag-NPs surface morphology revealed from AFM images show, size diameter 73.73 nm, Roughness average (Ra) and Root mean square (Sq) of was 14.7 nm, 18.2 nm, respectively. The FTIR spectrum of biosynthesized Ag-NPs showed six distinct peaks, 3711.87, 1699.18, 1108.81, 834.71, 446.70 and 419.33 cm-1. And altogether Candida spp. isolates remained vulnerable towards Ag-NPs and inhibition rate increases with the increase of concentration.
Conclusion: Biological synthesis of Ag-NPs using F.oxysporum have potent antimicrobial action in contradiction of Candida lambica, C.famata, C.glabrata, C.albicans, C.tropicalis, C.sphaerica and C.krusei.