STAINING OF LEISHMANIA DONOVANI PROMASTIGOTES BY NATURAL FLOWER DYES

Abstract

There are many synthetic dyes used to stain leishmania parasite for the purpose of diagnosis and the most famous are geimsa stain and leishman stain. Due to the damage caused by the use of synthetic dyes, including large environmental pollution and harmful toxic side effects this study came for the purpose of obtaining alternative dyes for the staining of the parasite from natural sources available, cheap and easy to obtain. The water extract of kujarat flowers Hibiscus sabdariffa and the alcoholic extract of Damask rose Rosadamascena and China rose Hibiscus rosa sinensis were prepared and used in staining the Leishmania donovani promastigotes and compared with geimsa stain. The three natural flower extracts gave a good capability in staining the promastigote of Leishmania donovani