HISTOPATHOLOGICAL EFFECT OF AZATHIOPRINE ON LIVER, INTESTINE AND SPLEEN OF ALBINO MICE

Abstract

This study was carried out to study the effect of azathioprine drug on some histological structure in albino mice. sixty adult female albino mice (20mice/group), were used in this study, the average body weights are 30-32 gm. two doses of azathioprine (50 mg/kg/b wt. and 100 mg/kg/b wt.) were given by the oral route daily for 40 days to the second and third group respectively, while control group was given normal saline. The histopathological examination of the treated animal for liver showed congestion with dilation of portal vein,
Dilation of sinusoids and Mono-nuclear cell aggregation for the second group while for the third treated group showed small granulomatous lesion of mononuclear cells, Enlargement and proliferation of kupper cells, Severe necrosis. Sever vacuolar degeneration of hepatocytes. The histopathological section of spleen was showed Infiltration of megakaryocyte for the second group while for the third group showed hypertrophy of central arteriole with dilation and infiltration of mono-nuclear cell mainly macrophage and plasma cell in red pulp. The histological changes in intestine tissue of the treated animals showed vacular degeneration of epithelial cell with hyperplasia of goblet cell which produce allots of mucin for the second group while for the third group showed infiltration of mononuclear cells between glands. From this study we concluded that there was harmful effect of azathioprine on different tissues of the experimental animal dependent on increasing dose of this drug.