TLR2 AND TLR4 AS A BIOMARKER IN ADULT AND CHILDREN IRAQI BACTERIAL SEPSIS SYNDROME PATIENTS

Abstract

This study aimed to investigate the possibility using of TLR2 and TLR4 gene expression as an early biomarkers for diagnosis bacterial septic syndrome in children and elderly. The causative agents of infection was determined by blood culture. TNF-α, IL-10, and sHLA-DR were measured by ELISA and TLR2, TLR4 expression was determined by quantitative RT-PCR. about 75 patients was diagnosed with sepsis syndrome included in this study . The age range of patients (13days-92years) with mean 56.3±13.9 and matched to 55 healthy volunteers. Depending on age, patients were divided into 4 age groups (group A <1 year, group B 1-13 year, group C 14-40 year and group D >40 year). The results indicated that the levels of TNF-α, IL-10 and sHLA-DR in all age groups were significantly increased except the level of TNF-α in group B compared with control groups. A positive correlation has observed between levels of TNF-α and sHLA-DR with patients age respectively. TLR2 and TLR4 mRNA expression was significantly increased in all age groups with significant difference between group A and groups D. TLR2 expression highly increases in G+ve infection while TLR4 expressed highly in G-ve bacteria. can be conclude that TLR2 and TLR4 expression in bacterial sepsis patients indicates the strong possibility of using them as biomarkers in the early diagnosis of bacterial sepsis syndrome, in children and elderly patients