OPTIMIZATION OF BACTERIOCIN PRODUCTION FROM Lactobacillus plantarum IN05 BY USING RESPONSE SURFACE METHODOLOGY

Abstract

Bacteriocins are ribosomally synthesized antimicrobial compound. Response surface methodology (RSM) is a combination of statistical and mathematical techniques used to create model and to analyze a response that is influenced by several factors. The objective of this study was to determine the optimum condition using RSM to obtain optimal bacteriocin production from Lactobacillus plantarum. Activity test was performed using well diffusion agar method. The four experimental factors, i.e. glucose concentration, pH, temperature and concentration of yeast extract with 30 treatment. The results indicated that the optimum condition of bacteriocin production from L. plantarum IN05 showed optimum inhibition activity against Salmonella typhimurium and Listeria monocytogenes, with the addition of 4 g/L glucose, 8.11 g/L yeast extract, pH of 5.3, and temperature of 30oC. The highest bacteriocin activities against S. typhimurium and L. monocytogenes were 3136 AU/mL and 2426 AU/mL, respectively.