MOLECULAR ANALYSIS OF RUST RESISTANCE IN SELECTED SUGARCANE CULTIVARS

Abstract

The present study aimed at to identify rust resistance based on DNA marker linkage among commercially sugarcane cultivar of Pakistan. Therefore primer were designed by using reported sequences of Resistance Gene analogues DNA and also universal Random Amplified Polymorphic DNA marker were select/picked from literature, initially, In selected rust resistant and susceptible sugarcane cultivar The Resistance Gene analogous marker shown 52 trackable loci and 76.92% polymorphism and the size of loci are between 100-200bp.While the Random Amplified Polymorphic DNA primer shown 100% polymorphism and 50 trackable loci were recorded and the size of loci are between 200-3000bp, out of eleven primers seven primer produced fourteen specific loci in three sugarcane genotypes, and the Random Amplified Polymorphic DNA Polymerase chain Reaction is important use for identifying Saccharum. Spp. By exploiting bivariant data of two different kind of markers (RGA and RAPD), the homology tree was constructed using DNAMAN 5.2.2 software based on Nei’s genetic distance and two major discrete groups with different minor groups were recorded for six genotypes having different disease behavior. This research work will be helpful for identifying resistance and susceptible sugarcane cultivars.