OPTIMIZATION OF PROCESS PARAMETERS AND SCALE-UP OF XYLANASE PRODUCTION USING CORN COB RAW BIOMASS BY MARINE BACTERIA BACILLUS SUBTILIS LBF M8 IN STIRRED TANK BIOREACTOR

Abstract

Optimization parameter for production several xylanases have already been studied, but only a few derived from marine bacteria especially Bacillus sp. Focus on this research is optimation of process parameters for xylanase production and scale-up production in stirred tank bioreactor using corn cob raw biomass as a substrate. Maximum xylanase production was obtained at 1,5% corn cob concentration, pH medium 6, temperature fermentation 30C, and agitation rate 200 rpm. For production using agricultural waste, production with corn cob (2,963 U/mL) is higher than rice straw (1,938 U/mL) and sugarcane bagasse (1,728 U/mL). Optimization process parameters increased xylanase activity from 1,864 U/mL at 96 h to 2,963 U/mL at 24 h incubation. The scale-up of the fermentation process up to 2 L stirred tank bioreactor with the optimum condition at flask scale, significantly increased xylanase activity to 3,519 U/mL.