DETECTION OF CASP-5 GENE AS INFLAMMATORY FACTOR IN IRAQI PATIENTS WITH PSEUDOMONAS AERUGINOSA INFECTIONS

Abstract

Pseudomonas aeruginosa is define as one of an aerobic Gram-negative bacteria that considered as one of most problematic nosocomial emerged pathogens. This study was performed on 50 isolates and blood patients. Definitive diagnosis of positive P. aeruginosa isolates based on the characteristic examination, the phenotypic of colonies and microscopic morphology which grown in media
also biochemical tests for diagnosis of isolates Fifty isolates identified as Pseudomonas aeruginosa, the percentage of isolates according to clinical sources were 19(38%) from burn, 12 (24%) from urine,3(6%) from ear swabs,10(20%) from sputum and 6(12%) from wounds. The diagnosis was conducted and confirmed by using API20E the result of it is 47(94%). The results showed isolates a different ability to resist antibiotics. Amoxicillin- clavulanic acid, Trimethoprim-sulfamethoxazole and Erythromycin were the effectiveness antibiotic against P. aeruginosa 100%.The ability of biofilm formation as a virulence factor, Micro-titer plate method (MTP) was used, results revealed that (46) isolates (92%) of P. aeruginosa have the ability to form biofilm by MTP method. Genotypic detection was done by conventional PCR for 16SrDNA gene by using specific primer (pa-ss) 50(100%) to detect the virulence gene (Flic gene) which is virulence factor gene in p. aeruginosa responsible for activation of caspase-1enzyme, the result showed 45(90%) positive. all the blood sample the results were positive %100 . Sequencing for 20 casp-5 PCR product to detect variations in gene of Iraqi population and the results showed 20 sample from 20 have a genetic variation, heterozygous nucleotide.