IDENTIFICATION OF MORPHOLOGICAL AND MOLECULAR VARIATION INDUCED BY GAMMA IRRADIATION ON MUSA CV. PISANG TANDUK (AAB)

Abstract

The Banana and plantain improvement through conventional breeding method has been very slow mainly due to narrow genetic variability, sterility and polyploid nature of most banana cultivars. Thus, mutation breeding is a promising tool to create new cultivars for the improvement of banana. Therefore, gamma treated banana plantlets were subjected to acclimatization under nursery and several study was conducted. Morphological study on the growth of mutated plantlets showed that, 10 Gy induced a significant stimulation effect on root length, leaf length and leaf width compared to non-treated banana. In the meanwhile, 30 Gy treatment induced dwarf plant. IRAP molecular analysis revealed that, the total of 37 DNA bands position were detected, out of them 20 were polymorphic, producing 54% polymorphism. The highest IRAP bands were detected using primer Sukkula LTR with a maximum number of polymorphic bands, whereas primer LTR 6149, Sukkula LTR/LTR 6149, Sukkula LTR/3'LTR and Sukkula LTR/5'LTR1 yielded monomorphic bands. Hence, in overall application of 10 Gy of γ induced the significant higher phenotypic and genotypic variation. Therefore, the current research was conducted to study the effect of gamma radiation on the morphological and genetic variation in γ irradiated banana plant.