PRODUCTION OF PROTEASES BY STAPHYLOCOCCUS EPIDERMIDIS EFRL 12 USING COST EFFECTIVE SUBSTRATE (MOLASSES) AS A CARBON

Abstract

Staphylococcus epidermidis EFRL 12 capable of producing extracellular protease isolated from soil. The protease production was investigated in batch wise submerged fermentation. The protease production was studied with varying carbon sources with (0.5 and 1.0%) concentrations, peptone (0.25-1.5%) and different nitrogen sources (1.0%).The effect of sugarcane bagasse, rice husk, molasses and date syrup were checked along with pure carbon sources. The best production was obtained when microorganism was grown on mineral medium containing 1.0% molasses as carbon source and 1.0% ammonium nitrate as nitrogen source after 6 hours of incubation at 37°C and initial pH was adjusted to 7.5.